14-3-3β基因与胃癌淋巴结转移的相关性研究  

The research of correlation between 1 4-3-3βand lympho node metastasis in gastric cancer

作　　者：朱冠宇[1] 魏玉哲[1] 姚强[1] 李岩峰[1] 王宽[1] 薛英威[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院胃肠外科,150081

出　　处：《国际遗传学杂志》2016年第6期306-311,共6页International Journal of Genetics

摘　　要：目的：探讨敲减及过表达14-3-3β基因对胃癌细胞生物学行为的影响及临床病理改变。方法在 SGC 7901、MGC 803和 HGC 27胃癌细胞系中，利用 RNA 干扰技术抑制14-3-3β基因的表达，分别用 MTT 法、基底膜侵袭实验检测胃癌细胞的增殖和侵袭能力的变化。结果在 HGC27和 MGC803细胞系中，14-3-3β基因的缺失会影响细胞增殖（ P 值分别为0.000、0.000、0.000、0.000、0.000、0.001、0.000、0.005），14-3-3β基因表达缺失的MGC803胃癌细胞侵袭能力降低（ P 值分别为0.000、0.000）。结论14-3-3β基因参与了胃癌细胞增殖和侵袭。Objective To investigate the biological behavior and the clinicopathological signifi -cance with silencing of 1 4-3-3βexpression in gastric cancer .Methods RNA interference method was used to knock-down the expression of 1 4-3-3βin gastric cell lines , and MTT method was used to detect tumor cell proliferation .We used transwell test to check the invasive ability of gastric tumor cells without 1 4-3-3βexpression .Results RNA interference could downregulate the endogenous 1 4-3-3βexpression efficiently in MGC 8 0 3 and HGC 2 7 cell lines .After transient transfection in HGC 2 7 and MGC 8 0 3 cell lines .The proliferation rate of transfected cells was significantly decrease compared to the negative control （ P values are 0.0 0 0 , 0.0 0 0 , 0.0 0 0 , 0.0 0 0 , 0.0 0 0 , 0.0 0 1 , 0.0 0 0 , 0.0 0 5 ） .Furthermore , the transwell chamber test showed that the absence of 1 4-3-3βexpression in MGC 8 0 3 could reduce the invasive capability of cancer cells （ P values are 0.0 0 0 , 0.0 0 0 ） . Conclusion RNA interference method is effective in koncking-down 1 4-3-3βexpression in gastric carcinoma cell line .1 4-3-3βmay be involved in regulating cell proliferation and invasion of gastric cancer .

关 键 词：14-3-3β基因 胃癌 淋巴结转移 

分 类 号：R735.2[医药卫生—肿瘤]