miR-128b对胶质母细胞瘤增殖、迁移和侵袭的影响  

Effect of miR-128bon Proliferation,Migration and Invasion of Glioblastoma Cells

作　　者：张章[1] 熊左隽[1] 范明波 陈文[1] Zhang zhang Xiong Zuoiun Fan Mingbo et al(Department of Neurosurgery , The Central Hospital of Wuhan , Tongji Medical College, Huazhong University of Science and Technology ,Wuhan 430014 ,China)

机构地区：[1]华中科技大学同济医学院附属武汉中心医院神经外科,武汉430014

出　　处：《华中科技大学学报（医学版）》2016年第6期608-612,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的研究miR-128b对胶质母细胞瘤增殖、迁移及侵袭的影响。方法 Real-time PCR测定miR-128b在胶质母细胞瘤细胞系U251及正常星形胶质细胞系HA1800中的表达。将U251细胞分为3组,即miR-128b类似物组、miR-128b抑制剂组及对照组,分别转染miR-128bmimics、抑制剂及microRNA scramble。转染24h后,Real-time PCR检测3组细胞中miR-128b的表达水平。通过MTT实验、克隆形成实验测定细胞增殖;通过细胞划痕实验、Transwell小室基质渗透实验测定细胞迁移及侵袭能力。结果miR-128b在胶质母细胞瘤细胞系U251较正常星形胶质细胞系HA1800低表达,为HA1800的(0.21±0.03)倍(P<0.01)。转染24h后,miR-128b类似物组miR-128b较对照组表达量显著升高,为对照组的(9.16±0.85)倍(P<0.01),miR-128b抑制剂组较对照组表达量显著降低,为对照组的(0.32±0.03)倍(P<0.01)。MTT结果示:miR-128b类似物组A490nm值在24、48和72h显著低于对照组和miR-128b抑制剂组;培养72h后,miR-128b类似物组克隆形成率为(24.67±2.82)%,对照组为(52.48±5.86)%,miR-128b抑制剂组为(82.63±9.14)%,3组比较,miR-128b类似物组的克隆形成率显著低于对照组及miR-128b抑制剂组(P<0.05,P<0.01)。miR-128b类似物组、miR-128b抑制剂组及对照组划痕愈合率分别为(31.24±3.72)%、(88.74±7.52)%及(62.37±4.33)%,miR-128b类似物组的划痕愈合率显著低于对照组及miR-128b抑制剂组(P<0.05)。miR-128b类似物组、miR-128b抑制剂组及对照组侵袭细胞数分别为每个100倍视野下(278±28)个、(696±43)个及(463±37)个,miR-128b类似物组的迁移细胞数显著少于对照组及miR-128b抑制剂组(均P<0.05)。结论 miR-128b抑制胶质母细胞瘤增殖、迁移及侵袭,有望作为一个新的治疗靶点。Objective To investigate the potential role of miR-128 bin regulation of glioblastoma cell proliferation,migration and invasion.Methods Expression level of miR-128 bin glioblastoma cell line U251 and normal astrocyte cell line HA1800 was investigated by real-time PCR.U251 cell line was divided into three groups：control group（transfected with microRNA scrambles）,miR-128bgroup（transfected with miR-128bmimics）and miR-128 binhibitor group（transfected with miR-128binhibitor）.MTT method and colon-formation assay were used to examine the cell proliferation ability.Cell scratch assay and Transwell assay were used to determine cell migration ability.Results Real-time PCR results indicated that the expression level of miR-128 bin U251was（0.21±0.03）folds the expression in HA1800（P〈0.01）.The gene expression of miR-128 bin miR-128 b mimics group was（9.16±0.85）times that of the control group（P〈0.01）,and miR-128 bexpression of miR-128 binhibitor group was（0.32±0.03）folds that of the control group 24 hafter transfection（P〈0.01）.The A490 nm of miR-128 bgroup was significantly lower than that of control group and miR-128 binhibitor group after culturing for 24,48 and 72h.After 72 hcultivation,the colony formation rate in miR-128 bgroup was（24.67±2.82）%,significantly lower than the rate [（52.48±5.86）%]in control group,and the rate[（82.63±9.14）%]in miR-128 binhibitor group（P〈0.05,P〈0.01）.The wound healing rate was significantly lower in miR-128bgroup[（31.24±3.72）%]than in miR-128 binhibitor group [（88.74±7.52）%]and control group[（62.37±4.33）%]（both P〈0.05）.The invasive cell number in miR-128 bgroup was（278±28）,significantly less than（696±43）in miR-128 inhibitor group and（463±37）in control group（P〈0.05）.Conclusion miR-128 binhibits glioblastoma cell proliferation,migration and invasion,and might be a new potential therapy target.

关 键 词：miR-128b 胶质母细胞瘤 增殖 迁移 侵袭 

分 类 号：R739.41[医药卫生—肿瘤]