肠淋巴液引流对失血性休克小鼠心肌组织ACE/ACE2的作用  

Role of mesenteric lymph drainage in the balance of ACE / ACE2 in murine myocardium following hemorrhagic shock

作　　者：王紫监[1,2] 刘俊芬[1,3] 蒋丽娜[1] 张立民[1] 刘桂青[1] 王淮淮 赵自刚[1] 牛春雨[1] WANG Zi-jian LIU Jun-fen JIANG Li-na ZHANG Li-min LIU Gui-qing WANG Huai-huai ZHAO Zi-gang NIU Chun-yu(Institute of Microcirculation of Hebei North University, Zhangjiakou 075000 the Second Affiliated Hospital of Hebei North University, Xuanhua 075100 the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Chin)

机构地区：[1]河北北方学院微循环研究所,张家口075000 [2]河北北方学院附属第二医院,宣化075100 [3]河北北方学院附属第一医院,张家口075000

出　　处：《中国应用生理学杂志》2017年第1期61-65,共5页Chinese Journal of Applied Physiology

基　　金：河北北方学院创新人才培育基金(CXRC1314)

摘　　要：目的:观察失血性休克后小鼠心肌组织血管紧张素转换酶(ACE)/ACE2平衡的变化及肠淋巴液引流(PHSML)的作用。方法:BALB/c雄性小鼠24只,随机分为对照组、假手术组、休克组、休克+引流组(n=6)。建立失血性休克模型,行液体复苏;休克+引流组液体复苏后,引流肠淋巴液。在液体复苏后6 h或假手术组相应时间点、对照组于麻醉后,留取心肌组织,q RT-PCR法检测ACE、ACE2、血管紧张素II(Ang II)1型受体(AT1R)、Mas相关G蛋白偶联受体(Mas1R)的mRNA表达,ELISA方法检测Ang II和Ang(1-7)含量。结果:休克组小鼠心肌组织ACE与AT1R mRNA表达、Ang II水平均显著高于对照组与假手术组,ACE2与Mas1R mRNA表达显著低于对照组与假手术组、Ang(1-7)含量显著低于对照组,ACE/ACE2、Ang II/Ang(1-7)、AT1R/Mas1R显著高于对照组与假手术组;PHSML引流显著抑制了失血性休克对这些指标的作用。结论:失血性休克上调心肌ACE-Ang II-AT1R轴、下调ACE2-Ang(1-7)-Mas1R轴表达,引起ACE/ACE2失衡;PHSML引流下调ACE-Ang II-AT1R轴、上调ACE2-Ang(1-7)-Mas1R轴表达,在一定程度上维持了ACE/ACE2平衡。Objective： To observe the change of angiotensin converting enzyme（ ACE） and ACE2 in the murine myocardium followed hemorrhagic shock and the role of post-hemorrhagic shock mesenteric lymph（ PHSML） drainage. Methods： Twenty-four male mice were randomly divided into control,sham,shock,and shock ＋ drainage groups. A hemorrhagic shock model was established and then fluid resuscitation was performed to the mice in the shock and shock ＋ drainage groups,and the PHMSL was drained in the shock＋ drainage group after fluid resuscitation. After 6 h of resuscitation in the shock and shock ＋ drainage groups or corresponding time in the sham group,or after anesthesia in the control group,the myocardial tissues were harvested for the determination of the m RNA expressions of ACE,ACE2,angiotensin II（ Ang II） type 1 receptor（ AT1R）,and Mas related G protein coupled receptor（ Mas1R） using the method of q RT-PCR,and the levels of Ang II and Ang（ 1-7） using the method of ELISA. Results： In the myocardial tissue of shock group,the ACE and AT1 R m RNA expressions and Ang II level were significantly increased than those of the control and sham groups,the ACE2 and Mas1 R m RNA expressions were significantly decreased than that of the control and sham groups,the Ang（ 1-7）level was decreased compared with the control group,the ratios of ACE / ACE2,Ang II / Ang（ 1-7）,and AT1 R / Mas1 R in the shock group were significantly increased than the control and sham groups. Meanwhile,PHSML drainage obviously suppressed the effects of hemorrhagic shock on these indices. Conclusion： Hemorrhagic shock up-regulated the ACE-Ang II-AT1 R axis,down-regulated the ACE2-Ang（ 1-7）-Mas1 R axis,and induced the unbalance of ACE and ACE2 in myocardial tissue. PHSML drainage decreased the ACE-Ang II-AT1 R axis and increased the ACE2-Ang（ 1-7）-Mas1 R axis,resulted in the balance of ACE and ACE2.

关 键 词：失血性休克 肠淋巴液 引流术 心肌 血管紧张素转换酶 小鼠 

分 类 号：R364.1[医药卫生—病理学] R331.4[医药卫生—基础医学]