Ethanol Extract of Phellinus merrillii Protects against Diethylnitrosamine- and 2-Acetylaminofluorene-Induced Hepatocarcinogenesis in Rats  

作　　者：YANG Chun-hung CHANG Heng-yuan CHEN Yi-chuan LU Chia-chen HUANG Shyh-shyun HUANG Guan-jhong LAI Hsin-chih 

机构地区：[1]Graduate Institute of Biomedical Science,Division of Biotechnology,College of Medicine,Chang Gung University,Tao-Yuan,333,Taiwan,China [2]Applied Toxicology Division,Taiwan Agricultural Chemicals and Toxic Substances Research Institute,Council of Agriculture,Executive Yuan,Taichung,413,Taiwan,China [3]School of Post-Baccalaureate Chinese Medicine,Tzu Chi University,Hualien,970,Taiwan,China [4]Department of Respiratory Therapy,Fu Jen Catholic University,Sinhuang,Taipei,242,Taiwan,China [5]School of Pharmacy,College of Pharmacy,China Medical University,Taichung,404,Taiwan,China [6]School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources,College of Pharmacy,China Medical University,Taichung,404,Taiwan,China

出　　处：《Chinese Journal of Integrative Medicine》2017年第2期117-124,共8页中国结合医学杂志（英文版）

基　　金：Supported by China Medical University Tsuzuki Institute for Traditional Medicine(No.CMU95-PH-11 and No.CMU97-232);the National Science Council(No.NSC 95-2320-B-182-059-MY3);Chang Gung University(No.CMRPD160271),Taiwan,China

摘　　要：Objective： To study whether the ethanol extract of Phellinus merrillii（EPM） has chemopreventive potential against liver carcinogenesis. Methods： Thirty male Spraque-Dawley rats were randomly divided into control group, EPM control group, hepatocarcinoma control group, low-dose EPM group and high-dose EPM group, 6 in each group. Using the Solt and Farber protocol in a rat model of hepatocarcinogenesis, the chemopreventive effect of EPM on diethylnitrosamine（DEN）-initiated, 2-acetylaminofluorene（2-AAF） and partial hepatectomy（PH）-promoted liver carcinogenesis in rats was evaluated. Basic pathophysiological and histological examinations, together with the serum levels of glutamic oxaloacetic transaminase（s GOT）, glutamic pyruvic transaminase（s GPT） and gamma-glutamyl transpeptidase（γ-GT） were measured. Results： Treatment of EPM at the concentration of 2 g/kg body weight in the diet for 8 weeks clearly prevented the development of carcinogenesis and reduced the levels of s GOT, s GPT, and serum γ-GT of rats as compared with the hepatocarcinoma control group（P〈0.05 or P〈0.01）. These phenotypes were accompanied by a significant increase in natural killer cell activity. Conclusion： EPM showed a strong liver preventive effect against DEN＋2-AAF＋PH-induced hepatocarcinogenesis in a rat model.Objective： To study whether the ethanol extract of Phellinus merrillii（EPM） has chemopreventive potential against liver carcinogenesis. Methods： Thirty male Spraque-Dawley rats were randomly divided into control group, EPM control group, hepatocarcinoma control group, low-dose EPM group and high-dose EPM group, 6 in each group. Using the Solt and Farber protocol in a rat model of hepatocarcinogenesis, the chemopreventive effect of EPM on diethylnitrosamine（DEN）-initiated, 2-acetylaminofluorene（2-AAF） and partial hepatectomy（PH）-promoted liver carcinogenesis in rats was evaluated. Basic pathophysiological and histological examinations, together with the serum levels of glutamic oxaloacetic transaminase（s GOT）, glutamic pyruvic transaminase（s GPT） and gamma-glutamyl transpeptidase（γ-GT） were measured. Results： Treatment of EPM at the concentration of 2 g/kg body weight in the diet for 8 weeks clearly prevented the development of carcinogenesis and reduced the levels of s GOT, s GPT, and serum γ-GT of rats as compared with the hepatocarcinoma control group（P〈0.05 or P〈0.01）. These phenotypes were accompanied by a significant increase in natural killer cell activity. Conclusion： EPM showed a strong liver preventive effect against DEN＋2-AAF＋PH-induced hepatocarcinogenesis in a rat model.

关 键 词：hepatocarcinogenesis Phellinus merrillii diethylnitrosamine 2-acetylaminofluorene chemoprevention 

分 类 号：R735.7[医药卫生—肿瘤]