髓样分化因子88在宫颈鳞癌组织中的表达及临床意义  

Expression and significance of Myeloid differentiation factor 88 in cervical squamous cell carcinoma

作　　者：李婷[1] 朱建福[2] 毛瑛玉[1] 林茂华[1] 郑建明[3] 林妙春[3] 

机构地区：[1]福建医科大学附属闽东医院病理科,福建福安355000 [2]福建医科大学附属闽东医院骨科,福建福安355000 [3]福建医科大学附属闽东医院中心实验室,福建福安355000

出　　处：《临床和实验医学杂志》2017年第4期339-343,共5页Journal of Clinical and Experimental Medicine

基　　金：福建医科大学非直属附属医院科学研究发展专项基金项目(FZS13001Z);福建省青年科研课题(2013-1-48);宁德市科技计划项目(20130104)

摘　　要：目的探讨髓样分化因子88(My D88)在正常宫颈组织、宫颈上皮内瘤变(CIN)组织和宫颈鳞癌组织中的表达及临床意义。方法采用免疫组化SP法检测30例正常宫颈组织、45例CIN组织及55例宫颈鳞癌组织中Toll样因子受体4(TLR4)及My D88蛋白的表达,并研究其与宫颈癌患者临床病理特征之间的相关性。采用Western Blot法检测正常宫颈组织及宫颈鳞癌组织中TLR4及My D88蛋白的表达。结果免疫组化结果显示,TLR4及My D88在正常宫颈组织、CIN及宫颈鳞癌组织中阳性表达逐渐增强,组间相比差异有统计学意义(P<0.05)。Western Blot结果提示,与正常宫颈组织相比,TLR4及My D88在宫颈鳞癌组织中的蛋白表达水平明显上调,宫颈鳞癌组织中TLR4蛋白表达水平增加了284.89%(P<0.05),My D88蛋白表达水平增加了45%(P<0.05)。且TLR 4蛋白表达异常增高与肿瘤浸润程度及有无淋巴结转移相关(P<0.05),与不同临床分期及肿瘤分化均无显著差异(P>0.05);My D88的表达与组织学类型和病理分级无关(P>0.05),与患者有无淋巴结转移及临床分期也无显著相关性(P>0.05)。结论 My D88可能参与宫颈鳞状上皮的恶性转变过程,TLR 4可能通过My D88信号通路来参与宫颈癌的发生、发展。Objective To explore the expression and significance of Myeloid differentiation factor 88 protein（ My D88） in normal cervical,cervical intraepithelial neoplasia（ CIN） and cervical squamous cell carcinoma（ CSCC）. Methods The protein expression of TLR4 and My D88 were detected by immunohistochemistry in 30 cases of normal cervical,45 cases of CIN and 55 cases of CSCC,by western blot in normal cervical and CSCC. The correlation between the expression of TLR4 and My D88 and clinical significance of patients were analyzed. Results Immunohistochemistry showed that the expression of TLR4 and My D88 were significantly enhanced in CIN and CSCC than those in normal cervical tissues（ P〈0. 05）. Western Blot results showed that compared with normal cervical tissue,the expression of TLR4 protein in CSCC was increased by 284. 89%（ P〈0. 05）,and the expression of My D88 protein was increased by 45%（ P〈0. 05）. The expression of TLR4 protein was correlated with the degree of tumor invasion and lymph node metastasis（ P〈0. 05）,and it had no obvious difference with clinical stages and tumor differentiation（ P〉0. 05）. The expression of My D88 was not correlated with histological type and histological grade（ P〉0. 05）,and was not significantly correlated with lymph node metastasis and clinical stage（ P〉0. 05）. Conclusion These results indicate that My D88 may participate in the process of cervical squamous epithelial malignant transformation from TLR4 / My D88 signal pathway.

关 键 词：宫颈肿瘤 髓样分化因子MyD88 Toll样因子受体4 

分 类 号：R737.33[医药卫生—肿瘤]