重复经颅磁刺激联合嗅鞘细胞移植治疗脊髓损伤  

Combination of olfactory ensheathing cell transplantation and repetitive transcranial magnetic stimulation for the treatment of spinal cord injury

作　　者：姜泳 迟晓飞 邹喜军 慈元 姚琦 杨茂伟 

机构地区：[1]大连市骨科医院,辽宁省大连市116011

出　　处：《中国组织工程研究》2017年第1期98-102,共5页Chinese Journal of Tissue Engineering Research

基　　金：大连市科技发展计划支持项目(2013E15SF168)~~

摘　　要：背景:传统观念认为哺乳动物的脑和脊髓损伤后不能再生,脊髓损伤后功能恢复的预后希望很渺茫。目的:分析经颅磁刺激联合嗅鞘细胞移植治疗大鼠脊髓损伤的修复效果。方法:对80只大鼠进行脊髓损伤模型制备,随机等分成4组,胸髓横断损伤组、嗅鞘细胞移植组、重复经颅磁刺激组及联合组。各组大鼠行T10全椎板切除建立胸髓横断损伤动物模型,造模24 h后,嗅鞘细胞移植组和联合组大鼠在损伤区附近注射嗅鞘细胞,重复经颅磁刺激组及联合组大鼠于左前囟上方接受重复经颅磁刺激治疗。结果与结论:移植4周后,与其他3组相比,重复经颅磁刺激联合嗅鞘细胞移植组大鼠运动功能恢复明显,运动功能BBB评分升高,坐骨神经电生理功能明显恢复,损伤脊髓修复较好。提示重复经颅磁刺激联合嗅鞘细胞移植治疗大鼠脊髓损伤可以获得显著疗效,两者联合应用可明显促进脊髓损伤后的轴突再生及功能恢复。BACKGROUND： Conventional wisdom suggests that the mammalian brain and spinal cord cannot be regenerated after injury and the hope for functional recovery is slim after spinal cord injury. OBJECTIVE： To test and analyze the therapeutic effect of olfactory ensheathing cell transplantation combined with repetitive transcranial magnetic stimulation （rTMS） in spinal cord injury rats.METHODS： Eighty rats were used to make spinal cord injury models by T10 laminectomy and then rat models were equivalently randomized into model, cell transplantation, rTMS, and combined treatment groups. Twenty-four hours after modeling, rats in the latter three groups were given olfactory ensheathing cell transplantation around the injured region, rTMS above the left anterior bregma, or their combination, respectively. RESULTS AND CONCLUSION： At 4 weeks following transplantation, the motor function of rats in the combined treatment group was significantly improved, accompanied by increased Basso, Beattie and Bresnahan score and significantly improved sciatic nerve electrophysiological function. These findings indicate that the olfactory ensheathing cell transplantation combined with rTMS treatment achieves remarkable outcomes, and the combined treatment can significantly improve the axonal regeneration and functional recovery after spinal cord injury.

关 键 词：干细胞 移植 嗅鞘细胞 重复经颅磁刺激 脊髓损伤 BBB评分 神经电生理学检查 苏木精-伊红染色 嗜银染色 神经丝蛋白 免疫组化染色 

分 类 号：R394.2[医药卫生—医学遗传学]