高效液相色谱法测定蔬菜中β-胡萝卜素的不确定度评定  

Uncertainty Evaluation on Determination of β-carotene in Vegetables by HPLC

作　　者：王晓婧[1] 曹梦锦 张雪松[1] 王国栋[1] 王竹[1] WANG Xiao-jing CAO Meng-jin ZHANG Xue-song WANG Guo-dong WANG Zhu(National Institute for Nutrition and health, CDC/Key Laboratory of Trace Element Nutrition, National Health and Family Planning Commission of the People＇s Republic of China, Beijing 100050, China)

机构地区：[1]中国疾病预防控制中心营养与健康所/国家卫生和计划生育委员会微量元素重点实验室,北京100050

出　　处：《中国食物与营养》2017年第1期51-55,共5页Food and Nutrition in China

基　　金：十二五"国家科技支撑项目"功能食品资源优化和评价共性关键技术研究"(项目编号:2012BAD33B01);卫计委标准司"国家食品安全标准食品中β-胡萝卜素的测定方法研究

摘　　要：建立高效液相色谱法测定蔬菜中β-胡萝卜素的不确定度评定数学模型,计算β-胡萝卜素测量结果的不确定度。胡萝卜和菠菜中β-胡萝卜素的扩展不确定分别为0.684和0.444 mg/100g(k=2、P=95%)。影响β-胡萝卜素HPLC法测定的不确定度来源主要是样品均匀性、前处理过程及HPLC仪器定量重复性,不确定度评定有利于完善测定结果的准确性。A mathematical model was established for determining β-carotene in vegetables by HPLC and the measurement uncertainty was evaluated. The expand uncertainties of β-carotene were 0. 684 mg/100g（ k = 2,P = 95%,for carrot） and 0. 444 mg/100g（ k = 2,P= 95%,for spinach）,respectively. The uncertainty from sample uniformity,pretreatment and HPLC instrument were the main sources of uncertainty and uncertainty evaluation was helpful to promote the accuracy of measurement.

关 键 词：不确定度 Β-胡萝卜素 蔬菜 高效液相色谱法 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]