MicroRNA-222对肺腺癌细胞增殖、迁移和侵袭的影响  

MicroRNA-222 promotes proliferation, migration and invasion of human lung adenocarcinoma cells by directly targeting ETS1

作　　者：卢滨[1] 贾金广 李利华[1] 姚菲菲[1] 

机构地区：[1]河南省郑州人民医院呼吸内科,河南郑州450003

出　　处：《中国现代医学杂志》2017年第3期34-40,共7页China Journal of Modern Medicine

摘　　要：目的探讨microRNA-222(miR-222)对肺腺癌细胞增殖、迁移和侵袭的影响。方法将miR-222和对照分别转染入肺腺癌细胞系A549进行活细胞计数法试剂盒(CCK-8)增殖实验、Transwell迁移及Matrigel侵袭实验,观察miR-222对肺腺癌细胞增殖、迁移和侵袭的影响。运用荧光素酶报告实验、实时荧光定量聚合酶链反应(qRT-PCR)及Western blot验证miR-222靶向下调ETS1的过程。结果 CCK-8增殖实验,Transwell迁移及Matrigel侵袭实验发现,与对照组相比miR-222能够促进肺腺癌细胞增殖、迁移和侵袭。荧光素酶报告实验、qRT-PCR及Western blot实验发现,miR-222可以靶向下调ETS1的表达,且ETS1参与调节上述过程。结论 miR-222通过靶向下调ETS1,促进肺腺癌细胞增殖、迁移和侵袭。Objective To investigate the role of microRNA-22（miR-222） in the proliferation, migration and invasion of human lung adenocarcinoma cells. Methods Firstly, miR-222 and negative control were transfected into lung adenocarcinoma cells, and the CCK-8 assay, Transwell migration and Matrigel invasion assays were performed to detect the effect of miR-222 on cell proliferation, migration and invasion respectively. Then the luciferase reporter assay, quantitative real-time polymerase chain reaction（qRT-PCR） and Western blot were performed to validate the putative target of miR-222. Subsequently, loss-of-function assay was applied to determine the target involved in the regulation of proliferation, migration and invasion of lung adenocarcinoma cells. Results The results of CCK-8 assay, Transwell migration and Matrigel invasion assays indicated that miR-222 could promote the proliferation, migration and invasion of lung adenocarcinoma cells. The luciferase reporter assay, qRT-PCR and Western blot showed that miR-222 directly targeted the 3＇UTR of ETS1. And overexpression of ETS1 significantly inhibited the miR-222-induced proliferation, migration and invasion of lung adenocarcinoma cells. Conclusions miR-222 facilitates proliferation, migration and invasion of lung adenocarcinoma cells by repressing ETS1.

关 键 词：microRNA-222 ETS1 增殖 迁移 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]