三叶木通化学成分及其抗氧化活性  

Chemical constituents from Akebia trifoliate and their anti-oxidant activities

作　　者：郭林新[1] 马养民[1] 乔珂[1] 李梦云[1] 王娜娜[1] 

机构地区：[1]陕西科技大学化学与化工学院,教育部轻化工助剂化学与技术重点实验室,陕西西安710021

出　　处：《中成药》2017年第2期338-342,共5页Chinese Traditional Patent Medicine

基　　金：国家公益性行业(林业)科研专项(200904004)

摘　　要：目的研究三叶木通Akebia trifoliata(Thunb.)Koidaz化学成分及其抗氧化活性。方法 DPPH法筛选三叶木通95%乙醇提取物的抗氧化活性部位,通过硅胶、Sephadex LH-20、制备柱进行分离纯化,根据理化性质和波谱数据鉴定所得化合物的结构,再评价其抗氧化活性。结果乙酸乙酯部位抗氧化活性最显著,从中分离出8个化合物,分别鉴定为齐墩果酸(1)、6,7,10-三羟基-8-十八烯酸(2)、正三十四烷(3)、2,6-二氧甲基-1,4-苯醌(4)、β-谷甾醇(5)、香草酸(6)、咖啡酸(7)、秦皮乙素(8)。化合物7和8对DPPH自由基的清除能力较强,IC50分别为14.939μg/m L和15.631μg/m L。结论化合物3、4、6为首次从木通属植物中分离得到,而且化合物7和8抗氧化活性较好。AIM To study the chemical constituents from Akebia trifoliata （ Thunb. ） Koidaz and their anti-ox- idant activities. METHODS DPPH method was used for screening A. trifoliata 95% ethanol extract＇s fraction with anti-oxidant activity. The isolation and purification were performed by silica, Sephadex LH-20 and preparative column, the structures of obtained compounds were identified by physicochemical properties and spectral data. Then their anti-oxidant activities were evaluated. RESULTS Ethyl acetate fraction showed the most significant anti-oxidant activity. Eight compounds were isolated and identified as oleanolic acid （1）, 6, 7, 10-trihydroxy-8- octadecenoic acid （2） , tetratriacontane （3） , 2, 6-dimethoxy-1, 4-benzoquinone （4） , [3-sitosterol （5） , vanillic acid （6）, caffeic acid （7）, aesculetin （8）. Compounds 7 and 8 exhibited strong scavenging activities against DPPH free radical with the IC50 values of 14. 939 μg/mL and 15.631 μg/mL, respectively. CONCLUSION Compounds 3, 4 and 6 are isolated from genus Akebia for the first time, and compounds 7 and 8 have good anti-oxi- dant activities.

关 键 词：三叶木通 化学成分 抗氧化活性 分离鉴定 DPPH 

分 类 号：R284.1[医药卫生—中药学]