应用MR氢谱检测大鼠脑胶质瘤C6细胞放射损伤  

A study of radiation injury in rat C6 glioma cell line by ^1H-nuclear magnetic resonance spectroscopy

作　　者：李红霞[1] 崔谊[1] 徐延杰[1] 李福艳[1] 史文琦[1] 王建震 曾庆师[1] 

机构地区：[1]山东大学齐鲁医院放射科,250012

出　　处：《中华放射肿瘤学杂志》2017年第2期228-233,共6页Chinese Journal of Radiation Oncology

基　　金：国家自然科学基金（81372439）

摘　　要：目的应用高分辨率MR氢谱检测大鼠脑胶质瘤细胞系C6的放射损伤并初步探讨其机制。方法以C6细胞为研究对象，应用MR氢谱检测细胞内多种代谢物浓度；彗星实验检测DNA损伤；流式细胞术检测周期进程及凋亡率；克隆形成实验观察克隆形成率并初步探讨放射损伤作用机制。采用单因素方差分析，Pearson法相关分析。结果照射剂量由0Gy增加为1、5、10、15Gy时，DNA损伤逐渐加重且有剂量依赖性（P=0．000～0．690），G1期所占百分比显著增加（P=0．026—0．749），凋亡率逐渐上升（P=0.000～0．000），克隆形成率逐渐降低（P=0．000—0．004）；同时，代谢物比值Lac／Cr逐渐减少（P=0．000-0．015），与DNA损伤参数（尾长、彗尾中DNA含量、尾矩）呈线性负相关（r=-0．971、-0．998、-0．995），与凋亡率呈线性负相关（r=-0．978）。结论MR氢谱检测发现C6细胞照射后Lac／Cr比值变化与肿瘤细胞凋亡有明显相关性，MR氢谱具有预测脑胶质瘤放射损伤的潜力。Objective To study the radiation injury of rat C6 glioma cell line by high resolution, ^1H-nuclear magnetic resonance （^1H NMR ） spectroscopy, and to preliminarily investigate its mechanism. Methods Metaholite concentrations in C6 cells were determined by ^1H NMR spectroscopy. Comet assay was used to evaluate DNA damage. Flow eytometry was used to determine the cell cycle and apoptosis rate. Colony-forming assay was used to measure the colony-forming rate and preliminarily investigate the mechanism of radiation injury. The results were analyzed by one-way analysis of variance and Pearson correlation analysis. Results With the increase in radiation dose from 0 Gy to 1, 5, 10, and 15 Gy, DNA damage was enhanced in a dose-dependent manner （P= 0. 000-0. 690） ;the percentage of cells in G1 phase increased （P = 0. 026-0. 749） ;the apoptosis rate significantly increased （ all P = 0. 000） ;the colony-forming rate significantly declined （ P = 0. 000-0. 004） ;the Lae/Cr ratio significantly decreased （ P = 0. 000-0. 015 ）, which had a negative linear correlation with DNA damage parameters （tail length, r=-0. 971 ;%DNA in the tail, r = - 0. 998 ; tail moment, r = - 0. 995 ） and apoptosis rate （ r = 0. 978 ）. Conclusions t H NMR spectroscopy reveals that the change in the Lac/Cr ratio is associated with injury and apoptosis of C6 cells after radiation. ^1H NMR spectroscopy has the potential to predict radiation injury of glioma.

关 键 词：脱氧核糖核酸损伤 磁共振氢谱 细胞系 胶质瘤 细胞凋亡 周期阻滞 

分 类 号：R739.41[医药卫生—肿瘤]