ApoB基因rs676210和rs2854725位点多态性与胆囊结石的相关性研究  

Association of apolipoprotein B gene rs676210 and rs2854725 polymorphisms with gallstone disease

作　　者：陈敏[1] 张涛[2] 克力木[3] 

机构地区：[1]新疆维吾尔自治区人民医院肛肠外科,乌鲁木齐830001 [2]新疆维吾尔自治区人民医院急救创伤外科,乌鲁木齐830001 [3]新疆维吾尔自治区人民医院微创疝腹壁外科,乌鲁木齐830001

出　　处：《中国普外基础与临床杂志》2017年第2期215-221,共7页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的探讨血浆载脂蛋白B(apolipoprotein B,Apo B)基因rs676210和rs2854725位点多态性与胆囊结石之间的关系以及其在民族间有无差异。方法采用病例对照研究的方法,收集2010~2014年期间在我院接受手术治疗的胆囊结石患者189例,其中维吾尔族99例,汉族90例;收集同期在我院进行健康体检者190例作为对照,其中维吾尔族93例,汉族97例。胆囊结石患者与健康对照者以及维吾尔族和汉族间的年龄和性别比较差异均无统计学意义(P>0.05)。采用SNa Pshot SNP分型技术进行Apo B基因分型,分析基因型分布在胆囊结石患者与健康对照者间以及维吾尔族和汉族间的差异。结果 1不同民族的胆囊结石患者和健康对照者间Apo B基因rs676210和rs2854725位点等位基因的分布频率比较差异均无统计学意义(维吾尔族:rs676210位点:χ~2=0.229,P=0.633;rs2854725位点:χ~2=0.028,P=0.866。汉族:rs676210位点:χ~2=0.608,P=0.435;rs2854725位点:χ~2=0.089,P=0.766)。2有无胆囊结石的维吾尔族和汉族间Apo B基因rs676210和rs2854725位点等位基因的分布频率比较差异均无统计学意义(胆囊结石患者:rs676210位点:χ~2=0.103,P=0.748;rs2854725位点:χ~2=3.139,P=0.076。健康对照者:rs676210位点:χ~2=0.000,P=0.990;rs2854725位点:χ~2=2.673,P=0.102)。3不同民族的胆囊结石患者和健康对照者间Apo B基因rs676210和rs2854725位点基因型的分布频率比较差异均无统计学意义(维吾尔族:rs676210位点:χ~2=2.301,P=0.317;rs2854725位点:χ~2=3.040,P=0.219。汉族:rs676210位点:χ~2=4.909,P=0.086;rs2854725位点:χ~2=0.107,P=0.744)。4在有胆囊结石的维吾尔族和汉族间Apo B基因rs676210和rs2854725位点基因型的分布频率比较差异均无统计学意义(rs676210位点:χ~2=0.235,P=0.899;rs2854725位点:χ~2=3.630,P=0.057)。在无胆囊结石即健康对照者中,rs676210位点的基因型分布频率在维吾尔族和汉族间总体比较差异无统计学意义(χ~2=1.026,P=0.599),而rs285472Objective To explore association ofapolipoprotein B （ApoB） gene rs676210 and rs2854725 polymorphisms with gallstone disease and differences of polymorphisms between Uygur population and Han population. Methods A case control study was used. One hundred and eighty-nine patients with gallstone disease from 2010 to 2014 in our hospital were collected, of which 99 cases of Uygur population and 90 cases of Han population. One hundred and ninety age- and sex-matched healthy volunteer accepted physical examination in our hospital over the same period were collected as control, of which 93 Uygur population and 97 Han population. The ApoB genotyping of DNA samples were amplified by using SNaPshot single nucleotide polymorphism （SNP）. The differences of polymorphisms between Uygur population and Han population and between patients with gallstone disease and healthy volunteer were analyzed. Results ①The differences of ApoB gene rs676210 and rs2854725 allele frequencies were not found between the patients with gallstone disease and healthy volunteer whether Uygur population or Han population （Uygur population： rs676210： χ^2=0.229, P=0.633; rs2854725： χ^2=0.028, P=0.866. Han population： rs676210： χ^2=0.608, P=0.435; rs2854725：χ^2=2.673, P=0.102）. ②The differences ofApoB gene rs676210 and rs2854725 allele frequencies were not found between Uygur population and Han population whether the patients with gallstone disease or healthy volunteer （Patients with gallstone disease： rs676210： χ^2=0.103, P=0.748; rs2854725： χ^2=3.139, P=0.076. Healthy volunteer： rs676210： χ^2=0.000, P=0.990; rs2854725：χ^2=2.673, P=0.102）. ③The differences of ApoB gene rs676210 and rs2854725 genotype frequencies were not found between the patients with gallstone disease and healthy volunteer whether Uygur population or Han population （Uygur population： rs676210： χ^2=2.301, P=0.317; rs2854725： χ^2=3.040, P=0.219. Han population： rs676210： χ^2=4.909, P=0.086; rs2854725： χ^2=0.107, P=0.74

关 键 词：血浆载脂蛋白B 胆囊结石 基因多态性 

分 类 号：R575.62[医药卫生—消化系统]