发卡型寡聚酰胺对双链DNA的识别研究  

Recognition study of double strand DNA with hairpin oligopolyamide

作　　者：李文佳[1] 石环环[1] 董波[1] 刘正春[1] LI Wenjia SHI Huanhuan DONG Bo LIU Zhengchun(Hunan Key Laboratory for Super Microstructure and Ultrafast Process, School of Physics and Electronics, Central South University, Changsha 410083, P.R.China)

机构地区：[1]中南大学物理与电子学院超微结构与超快过程湖南省重点实验,长沙410083

出　　处：《生物医学工程学杂志》2017年第1期134-139,共6页Journal of Biomedical Engineering

基　　金：国家自然科学基金(61371042);中南大学教师基金(2013JSJJ060)

摘　　要：对特定序列的双链DNA(ds DNA)的识别是近几十年来生物医学和分子生物学等领域一大研究热点。利用原有的核苷酸或人工设计的化学分子配体与ds DNA序列之间的特异性识别作用,基因疾病诊断、基因治疗、基因编辑可以被实现。发卡型寡聚酰胺是一种能在ds DNA螺旋小沟处以较好的亲和力特异性结合ds DNA序列的分子配体,具有良好细胞膜通透性、胞内稳定性等优点。本文综述了发卡型寡聚酰胺对ds DNA的识别性质及其在生物化学、生物医学等方面的应用,为发卡型寡聚酰胺的设计及应用提供参考。Selective recognition of double strands DNA （dsDNA） has been a research hot spot in molecular biology and biomedicine for a couple decades. Based on the selective interaction between natural nucleic acid/synthetic molecular ligands and dsDNA, gene diagnosis, gene therapy and gene editing would be realized. Hairpin oligopolyamide is a molecular ligand with excellent cellular permeability and nucleases-resistance which can target dsDNA sequence with high affinity and specificity at minor groove. This paper reviews the binding properties and biomedical applications of hairpin oligopolyamide targeting dsDNA, which provide references for further design and application of hairpin oligopolyamide.

关 键 词：发卡型寡聚酰胺 双链DNA识别 基因诊断 基因治疗 基因编辑 

分 类 号：Q78[生物学—分子生物学] O641.3[理学—物理化学]