Ⅱ型糖尿病肾病大鼠肾动脉与肾内小动脉钙化及BMP2和Runx2的表达  

Expression of BMP2 and Runx2 and vascular calcification in renal artery and arteriole of type 2 diabetic Nephropathy rats

作　　者：陈澄 覃春美 欧三桃 吴蔚桦 魏晓 龚财判 刘琦 CHEN Cheng QIN Chunmei OU Santao WU Weihua WEI Xiao GONG Caipan LIU Qi(Department of Nephrology , The Affiliated Hospital of Southwest Medical University ,Luzhou 646000, Sichuan, Chin)

机构地区：[1]西南医科大学附属医院肾病内科,四川泸州646000

出　　处：《西部医学》2017年第2期154-158,共5页Medical Journal of West China

基　　金：西南医科大学附属医院基金(泸医附院[2013]60号)

摘　　要：目的观察Ⅱ型糖尿病肾病(DN)大鼠肾动脉与肾内小动脉钙化以及骨形态发生蛋白2(BMP2)和成骨特异性转录因子2(Runx2)的表达。方法将24只大鼠随机分为对照组(N组)和Ⅱ型糖尿病肾病组(DN组,STZ加高脂高糖饮食诱导的Ⅱ型DN大鼠模型),分别于12周,16周处死大鼠。全自动生化分析仪检测大鼠血糖、尿素氮(BUN)、肌酐(Scr)、胱抑素C(CysC)及24h尿蛋白水平。CVon Kossa染色观察肾动脉及肾内小动脉钙盐沉积情况,免疫组化检测肾动脉及肾内小动脉BMP2及Runx2蛋白表达。结果 DN组大鼠各时间点血糖、尿素氮、胱抑素C、肌酐及24小时尿蛋白水平均较N组明显升高(均P<0.05)。Von Kossa染色可见N组大鼠肾动脉及肾内小动脉各时间点均无明显钙盐沉积,而DN组大鼠肾动脉第12周时即有黑色颗粒沉积,16周时黑色钙盐沉积进一步增多,但各时间点肾内小动脉均无明显黑色颗粒沉积。DN组大鼠肾动脉上可见BMP2及Runx2的较多表达,较N组明显升高(P<0.05);肾内小动脉也可见BMP2及Runx2少量表达,但与N组相比差异无统计学意义(P>0.05)。结论Ⅱ型糖尿病肾病模型大鼠早期肾动脉即可出现血管钙化,但肾内小动脉无明显钙化,提示糖尿病血管钙化较多发生在大血管,而不易发生在脏器内小动脉。Objective To observe the calcification and the expression of BMP2,Runx2 in renal artery as well as renal arteriole in type 2 diabetic nephropathy rats. Methods 24 male rats were randomly divided into control group （N group） and diabetic nephropathy group （DN group）. Type 2 diabetic nephropathy model was established by high sugar and fat diet and streptozotoein （STZ） injection. Calcium depositing in the renal artery as well as renal arteriole were observed by von Kossa staining. The protein expression of BMP2 and Runx2 were detected with immunohistochemistry. Results Contents of blood glucose, blood urea nitrogen（ BUN ）, cystatin C （Cys C）, Serum creatinine （Scr） and 24 h urinary protein （24 h Upro） of group DN at all time point were much higher than those of group N （P〈0.05）. Black granules were found deposited in the renal artery of DN rats in 12 and 16 weeks and the black calcium salt was gradually increased with the time passing. No calcium salt was found in group N. However, no calcium Salt was found in group N and group DN in the renal arteriole. The protein expression of BMP2 and Runx2 in group DN rats were significantly higher than that of N group in renal artery. The expression of BMP2 and Runx2 in the renal arteriole was little, which had no statistical significance from that of the normal group. Conclusion The renal artery calcification may appear in the early stages of type 2 diabetic nephropathy rats model, while there is no obvious calcification in the renal arteriole. It indicates that vascular calcification is more occurred in large blood vessels and less prone to occur in arteriole in the viscera.

关 键 词：糖尿病肾病 肾动脉 肾内小动脉 BMP2 RUNX2 

分 类 号：R587.2[医药卫生—内分泌]