Effects of Bacterial-Feeding Nematodes and Glucose on Phenanthrene Removal by Pseudomonas putida  

作　　者：JING Yongping LIYan LIU Zhaohui ZHANG Yingpeng LIU Ping SUN Ming Jiafa LUO 

机构地区：[1]Institute of Agricultural Resources and Environment, Shandong Academy of Agricultural Sciences, Jinan 250100 (China) [2]Key Laboratory of Agro-Environment in Huang-Huai-Hai Plain, Ministry of Agriculture, Jinan 250100 (China) [3]Shandong Provincial Key Laboratory of Plant Nutrition and Fertilizer, Jinan 250100 (China) [4]Shandong Academy of Agricultural Sciences, Jinan 250100 (China) [5]AgResearch, Hamilton 3240 (New Zealand)

出　　处：《Pedosphere》2017年第1期165-171,共7页土壤圈（英文版）

基　　金：supported by the Shandong Academy of Agricultural Sciences Youth Fund,China(No.2014QNM49);the National Key Technology R&D Program during the 12th Five-Year Plan Period (No.2012BAD15B02);the Program of Taishan Scholars for Overseas Experts,China;the Key Project of Science and Technology Innovation of Shandong Academy of Agricultural Sciences,China(No.2014CXZ01)

摘　　要：Bacterial-feeding nematodes can promote the bacterial activity through feeding.Bacterial abundance and their activity affect the degradation of polycyclic aromatic hydrocarbons(PAH) such as phenanthrene.The effects of bacterial-feeding nematodes,bacteria,and their interactions on the degradation of phenanthrene with or without glucose were studied through a microcosm experiment.The results showed that up to 57.0%of phenanthrene in mineral medium contaminated with phenanthrene was degraded in the control with bacteria alone and bacteria with the presence of nematodes and/or glucose increased the degradation of phenanthrene by 25.6%to 36.6%.Although both nematode and bacteria abundance decreased gradually,catechol 2,3-dioxygenase(C230) activity increased during the incubation period.Compared with bacteria alone,the presence of nematodes significantly increased C230 activity as well as the abundance of bacteria;this effect was more pronounced when glucose was present.The results imply that nematodes might promote the removal of phenanthrene from medium by stimulating bacteria and C230 activities.Bacterial-feeding nematodes can promote the bacterial activity through feeding. Bacterial abundance and their activity affect the degradation of polycyclic aromatic hydrocarbons （PAH） such as phenanthrene. The effects of bacterial-feeding nematodes, bacteria, and their interactions on the degradation of phenanthrene with or without glucose were studied through a microcosm experiment. The results showed that up to 57.0% of phenanthrene in mineral medium contaminated with phenanthrene was degraded in the control with bacteria alone and bacteria with the presence of nematodes and/or glucose increased the degradation of phenanthrene by 25.6% to 36.6%. Although both nematode and bacteria abundance decreased gradually, catechol 2,3-dioxygenase （C230） activity increased during the incubation period. Compared with bacteria alone, the presence of nematodes significantly increased C230 activity as well as the abundance of bacteria; this effect was more pronounced when glucose was present. The results imply that nematodes might promote the removal of phenanthrene from medium by stimulating bacteria and C230 activities.

关 键 词：BIODEGRADATION BIOSTIMULATION catechol 2 3-dioxygenase enzyme activity polycyclic aromatic hydrocarbons 

分 类 号：X172[环境科学与工程—环境科学]