ESM-1在低氧环境下胃腺癌细胞中的表达及意义  

Expression and significance of ESM-1 in hypoxia-induced human gastric cancer cells

作　　者：陈绍龙[1] 魏翔[1] 王怡[1] 周青[1] 张素梅[1] 汪渊[1] 

机构地区：[1]安徽医科大学分子生物学实验室、生物化学与分子生物学教研室、安徽省省部共建教育部重要遗传病基因资源利用重点实验室,合肥230032

出　　处：《安徽医科大学学报》2017年第1期23-27,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81201907);高校省级优秀青年人才基金项目(编号:2012SQRL067);安徽医科大学博士科研基金(编号:XJ201229)

摘　　要：目的研究用二氯化钴(CoCl_2)模拟胃癌细胞缺氧微环境的合适浓度,探讨内皮细胞特异性分子-1(ESM-1)在胃腺癌SGC-7901/BGC-823细胞缺氧环境中的表达情况。方法以两种人胃腺癌细胞(SGC-7901/BGC-823)为研究对象,分别用不同浓度二氯化钴(CoCl_2)处理人胃腺癌细胞后,用四唑化合物(MTS)法检测CoCl_2对SGC-7901和BGC-823细胞增殖的影响;Western blot分析不同浓度CoCl_2处理的人胃腺癌SGC-7901和BGC-823细胞中低氧诱导因子-1α(HIF-1α)和ESM-1表达的变化。结果≤0.6 mmol/L浓度的CoCl_2对胃腺癌SGC-7901和BGC-823细胞的增殖和细胞活力没有明显影响。在0.5、0.6 mmol/L CoCl_2浓度时,HIF-1α蛋白表达明显增加,但ESM-1表达明显下降。结论0.5、0.6 mmol/L CoCl_2可以模拟SGC-7901/BGC-823细胞的缺氧环境,在CoCl_20.5、0.6 mmol/L模拟细胞缺氧环境中,ESM-1的表达量与HIF-1α呈负相关。Objective To investigate the effect of CoC12-induced chemical hypoxia on proliferation of human gastric carcinoma SGC-7901 and BGC-823 cells, and to study the expression of ESM-1 in the different degrees of hypoxia gastric carcinoma cells. Methods SGC-7901/BGC-823 cells were treated with 0. 1,0. 2,0. 3,0. 5,0. 6 mmol/L CoC12 for 24 hours. The expressions of HIF-ltx and ESM-1 were detected by Western blot. The change of cell prolif- eration was detected by MTS. Results Cobalt dichloride at the concentration lower than 0.6 mmol/L had no sig- nificant effect on proliferation of gastric carcinoma cell line SGC-7901 and BGC-823 cells. The Western blot analysis illustrated that the expression of ESM-1 declined obviously as the expression of HIF-1 ct increased. Conclusion 0. 5,0. 6 mmol/L Cobalt dichloride can induce cell hypoxia. ESM-1 is negatively correlated with HIF-1α.

关 键 词：胃癌 缺氧 内皮细胞特异性分子-1 低氧诱导因子 

分 类 号：R735.2[医药卫生—肿瘤]