光甘草定对结直肠癌细胞RKO增殖和凋亡的影响  

Effects of glabridin on the proliferation and apoptosis of colorectal cancer cells

作　　者：杨小平[1] 邹多兵 丁延辉[1] 左莉[1] 周青[1] 汪渊[1] 

机构地区：[1]安徽医科大学分子生物学实验室、生物化学与分子生物学教研室,安徽省/省部共建教育部重要遗传病基因资源利用重点实验室,合肥230032

出　　处：《安徽医科大学学报》2017年第1期42-46,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81272399);安徽省自然科学基金(编号:1508085QH167)

摘　　要：目的研究光甘草定(Gla)对人结直肠癌细胞株RKO增殖和凋亡的影响,并初步探讨其可能的作用机制。方法选取RKO细胞为研究对象,采用MTT比色法测定Gla对RKO细胞增殖的影响,通过Hoechst 33258染色检测RKO细胞凋亡情况;Western blot法检测RKO细胞内丝裂原活化蛋白激酶(MAPK)信号通路蛋白以及B细胞淋巴瘤/白血病-2(Bcl-2)、B细胞淋巴瘤/白血病-2相关X蛋白(Bax)、半胱天冬氨酸蛋白酶-8(Caspase-8)、半胱天冬氨酸蛋白酶-9前体(Procaspase-9)、半胱天冬氨酸蛋白酶-3(Caspase-3)表达水平。结果 Gla抑制RKO细胞的增殖,并呈浓度与时间依赖性(P<0.05);Gla能调节MAPK信号通路JNK1/2,p38磷酸化的水平;Gla可诱导RKO细胞凋亡,下调抗凋亡蛋白Bcl-2、Procaspase-9的表达水平(P<0.05),上调Bax、Caspase-3蛋白的表达(P<0.05),而Caspase-8蛋白水平在各组变化均不明显。结论 Gla能抑制结直肠癌RKO细胞的增殖,并通过下调Bcl-2/Bax的比值诱导凋亡,其可能与MAPK信号通路的激活有关。Objective To explore the effects of glabridin on the proliferation of human colorectal cancer cell line RKO and the probable mechanisms. Methods MTY assay was used to detect the proliferation of RKO cells treated with different concentrations of glabridin. Hoechst staining was used to detect the apoptosis of RKO cells. The ex- pression of mitogen-activated protein kinase （MAPK） signaling pathway proteins and the proteins associated with apoptosis B-cell lymphoma-2 （ Bcl-2 ）, Bcl-2 assaciated X protein （ Bax）, cysteinyl aspartate specific proteinases （Caspase-8, Procaspase-9, Caspase-3） in RKO cells were detected by Western blot. Results Glabridin inhibited the proliferation of RKO cells in a dose- and time-dependent manner compared with cell group （ P 〈 0. 05 ）. Glabri- din regulated the expression of MAPKs. Glabridin induced the apotosis of RKO cells, down-regulated the expression of Bcl-2 and Procaspase-9 and up-regulated the expression of Bax and Caspase-3 compared with the control group （P 〈 0.05 ） , while Caspase-8 expression was constant in all groups. Conclusion Glabridin exhibits an inhibitory effect on RKO cells proliferation. Glabridin promotes the apoptosis of RKO cells, which may regulate the expression of the proteins associated with apoptosis through MAPK signaling pathway.

关 键 词：结直肠癌 RKO细胞 光甘草定 凋亡 

分 类 号：R735.3[医药卫生—肿瘤] R963[医药卫生—临床医学]