长链非编码RNAHOTAIR抑制膀胱尿路上皮癌对阿霉素的敏感性  

Long noncoding RNA HOTAIR inhibits the chemosensitivity to adriamycin of bladder urinary tract epithelial carcinoma

作　　者：张辉[1] 尚超[2] 郭艳[3] 宋永胜[1] 

机构地区：[1]中国医科大学附属盛京医院泌尿外科,辽宁沈阳110122 [2]中国医科大学基础医学院神经生物教研室,辽宁沈阳110122 [3]中国医科大学附属口腔医院,辽宁省口腔医学重点实验室,辽宁沈阳110122

出　　处：《现代肿瘤医学》2017年第5期673-676,共4页Journal of Modern Oncology

基　　金：国家自然科学基金(编号:81301834;81172408;30901480)

摘　　要：目的:研究长链非编码RNA HOTAIR在膀胱尿路上皮癌中的表达,明确其对膀胱尿路上皮癌对阿霉素敏感性的调控作用。方法:HOTAIR基因在膀胱尿路上皮癌及癌旁组织中的表达由实时定量PCR法检测。将HOTAIR基因的表达载体和沉默载体分别转染膀胱尿路上皮癌J82细胞,实时定量PCR验证转染效果。应用MTT法检测HOTAIR基因表达改变对于J82细胞的增殖能力及对阿霉素敏感性的调控作用。结果:HOTAIR基因在膀胱尿路上皮癌表达显著上调。HOTAIR基因的表达载体和沉默载体能够显著上调或沉默HOTAIR基因的表达。HOTAIR高表达能够促进J82细胞增殖,抑制J82细胞对阿霉素的敏感性;而HOTAIR表达沉默的J82细胞增殖能力下调明显,对阿霉素的敏感性明显增加。结论:长链非编码RNA HOTAIR在膀胱尿路上皮癌中高表达,HOTAIR能够作为癌基因促进膀胱尿路上皮癌的细胞增殖能力并抑制膀胱尿路上皮癌对阿霉素敏感性。Objective： To research the expression of lncRNA HOTAIR in bladder urinary tract epithelial carcinoma and its regulation for chemosensitivity to adriamycin. Methods： The expression of HOTAIR in bladder urinary tract epithelial carcinoma tissues and homologous peri-cancerous tissues were detected by fluorescence quantitive PCR. The expression vector and silence vector for HOTAIR were transfected into bladder urinary tract epithelial carcinoma J82 cells,and the transfected effects were detected by fluorescence quantitive PCR. The influence of HOTAIR expression changes on cell proliferation and chemosensitivity to adriamycinin in J82 cells were detected by MTT assay. Results：The results of fluorescence quantitive PCR discovered that,in contrast with homologous peri-cancerous tissues,the HOTAIR expressions increased markedly in bladder urinary tract epithelial carcinoma tissues. The expression and silence vector for HOTAIR could up-regulate and silence the HOTAIR expression respectively in J82 cells. In J82 cells,HOTAIR over-expression promoted cell proliferation,amd inhibited chemosensitivity to adriamycin. Silence of HOTAIR showed opposite regulative effects. Conclusion： Long noncoding RNA HOTAIR was high-expressed in bladder urinary tract epithelial carcinoma,it acted as an oncogene to advance cell proliferation,inhibite chemosensitivity to adriamycin in bladder urinary tract epithelial carcinoma cells.

关 键 词：膀胱尿路上皮癌 长链非编码RNA HOTAIR 阿霉素 

分 类 号：R737.14[医药卫生—肿瘤]