^125I放射性粒子离体照射对人舌鳞癌CAL-27细胞的抑制作用  

The inhibition effects of ^125I seed irradiation on human tongue squamous carcinoma cell line CAL -27

作　　者：邵翠玲[1] 孟箭[2] 王兴[2] 辜健敏 韩琨[1] 

机构地区：[1]潍坊医学院口腔医学院,山东潍坊261053 [2]徐州市中心医院口腔科,江苏徐州221009

出　　处：《徐州医学院学报》2017年第1期14-16,共3页Acta Academiae Medicinae Xuzhou

基　　金：江苏省“六大人才高峰”D类资助项目（2012-WS-099）;徐州市医学创新技术攻关团队（XWCX201604）

摘　　要：目的建立放射性粒子离体照射模型，观察^125I粒子持续低剂量率照射对人舌鳞癌CAL-27细胞的抑制效果。方法建立放射性粒子环形排布的照射模型，将指数生长期细胞分为4组：空白对照组、不同剂量粒子照射组（2、4、6Gy组），采用CCK-8法检测^125I粒子照射对人舌鳞癌CAL-27细胞的抑制率，倒置显微镜观察细胞学形态变化。结果2、4、6Cy粒子照射组所对应的细胞抑制率分别为（60．27±1．07）％、（71．48±0．65）％和（76．59±1．50）％，经方差分析不同照射剂量组间抑制率差异有统计学意义，呈时间和剂量依赖性（F=314．475，P〈0．01）。结论本方法建立的放射性粒子离体照射模型简便实用，可用于开展放射生物学实验，为研究^125I粒子持续低剂量率照射对舌鳞癌细胞的作用机制奠定了基础。^125I放射性粒子对CAL-27细胞生长具有显著的抑制作用。Objective To establish a model of ^125I seed irradiation in vitro and investigate its inhibitory effects on human tongue squamous carcinoma cell line CAL-27. Methods The radiation model of radiative particles in a circular array was established. The cells in the exponential phase were divided into four groups： a blank group and irradiation groups using various doses of radiative particles （ Groups 2, 4, and 6 Gy）. Then, CCK - 8 assay was performed to evalu- ate the inhibitory effects of ^125I seed irradiation on CAL- 27 cells. Meanwhile, the morphological changes were observed under an inverted phase contrast microscope. Results The inhibitory rate of Groups 2, 4, and 6 Gy was （60.27±1.07 ） % , （71.48±0.65 ） % and （76.59 ± 1.50） % , respectively. According to analysis of variance, these inhibito- ry rates were statistically different in a time - and dose - manner （F = 314. 475, P 〈 0.01 ）. Conclusions The radia- tion model of radiative particles is simple and efficient for radiobiological experiments, which lay foundations for studies a- bout the mechanism underlying the inhibitory effects of 125I seed irradiation on CAL -27 cells, ^125I seeds can remarkably inhibit the proliferation of CAL -27 ceils.

关 键 词：舌鳞状细胞癌 ^125I粒子 抑制作用 

分 类 号：R739.86[医药卫生—肿瘤]