米非司酮对人宫颈癌Hela细胞放疗敏感性的影响及作用机制  

Effects of mifepristone on the radiation sensitivity of humor cervical cancer Hela cells and related mechanisms

作　　者：魏敏 杜文升 陆晓媛 王侠 王世良 

机构地区：[1]徐州医科大学附属医院妇产科,江苏徐州221002

出　　处：《徐州医学院学报》2017年第1期21-24,共4页Acta Academiae Medicinae Xuzhou

基　　金：徐州市科技局立项支撑项目（KC14SH113）

摘　　要：目的探讨米非司酮对人宫颈癌Hela细胞放疗敏感性的影响及其相关作用机制。方法体外培养人宫颈癌Hela细胞，随机分为对照组、米非司酮组、放疗组、米非司酮＋放疗组，采用MTr法观察不同实验组Hela细胞的增殖抑制率，流式细胞技术分析Hela细胞凋亡率及细胞周期分布，Westernblot法检测Hela细胞中NF-κB p65蛋白表达水平。结果与其他各组相比，米非司酮＋放疗组Hela细胞的增殖抑制率、凋亡率、G0／G1期细胞比例均显著增加，S期、G2／M期细胞比例明显减少（P〈0．05或P〈0．01）。米非司酮＋放疗组Hela细胞中NF-κB p65蛋白表达水平较单纯放疗组明显下降（P〈0．01）。结论米非司酮可能通过NF-κB信号通路下调NF-κB p65蛋白表达、调节细胞周期、诱导Hela细胞凋亡等机制．增强宫颈痛细胞对放疗的敏感忡．Objective To investigate the effects of mifepristone on the radiation sensitivity of human cervical cancer Hela cells and its mechanisms. Methods Hela cells cultured in vitro were randomly divided into four groups： a control group, a mifepristone group, a radiotherapy group and a mifepristone ＋ radiotherapy group. The proliferation rates of Hela cells in each experimental group were observed by MTY. The apeptotic rate of Heia cells and cell cycle distribution were analyazed by flow cytometry. The level of NF-κB p65 was detected by Western blotting. Results Compared with other groups, the mifepristone ＋ radiotherapy group produced remarkably increases in the proliferation rate, apoptotic rate and the percentage of cells at the G0/G1 phase. In contrast, the percentage of cells in S and G2/M phases was markedly decreased. Compared with the radiotherapy group, the levels of NF-κB p65 were lower in the mifepristone ＋ radiotherapy group than those in the control group. Conclusions Mifepristone can enhance the radiation sensitivity of cervical cancer cells, which may be involved in adjusting cell cycle distribution and accelerating cell apoptosis by down - regulating the levels of NF -κB p65 protein in Hela cells through the NF -κB signaling pathway.

关 键 词：人宫颈癌 HELA细胞株 米非司酮 放射治疗 增殖抑制 凋亡 细胞周期 NF-κB P65 

分 类 号：R737.33[医药卫生—肿瘤]