改进的QuEChERS气相色谱-串联质谱法测定草莓中21种杀菌剂残留  

Residues of 21 fungicides in Fragaria ananassa by modified QuEChERS and gas chromatography-tandem mass spectrometry

作　　者：侯雪[1] 韩梅[1] 邱世婷[1] 胡莉[1] 雷绍荣[1] HOU Xue HAN Mei QIU Shiting HU Li LEI Shaorong(Laboratory of Quality and Safety Risk Assessment for Agro-products （ Chengdu）, Ministry of Agriculture/Center of Analysis and Testing Sichuan Academy of Agricultural Sciences, Chengdu 610066, China)

机构地区：[1]四川省农业科学院分析测试中心/农业部农产品质量安全风险评估实验室(成都),成都610066

出　　处：《农药学学报》2017年第1期46-52,共7页Chinese Journal of Pesticide Science

摘　　要：采用改进的Qu ECh ERS气相色谱-串联质谱法建立了检测草莓中21种杀菌剂(螺环菌胺、甲霜灵、四氟醚唑、酞菌酯、嘧菌环胺、戊菌唑、氟吡菌酰胺、氟菌唑、嘧菌胺、E-苯氧菌胺、抑霉唑、腈菌唑、醚菌酯、环氟菌胺、肟菌酯、丙环唑、环酰菌胺、氟环唑、咪唑菌酮、啶酰菌胺、嘧菌酯)残留的方法。样品经乙腈提取,采用N-丙基乙二胺(primary secondary amine,PSA)和石墨化碳黑(graphitized carbon blacks,GCB)作为分散固相萃取(dispersive solid phase extraction,DSPE)的吸附材料,净化液经氮气吹干、用V(正己烷):V(丙酮)=9:1混合溶液定容,采用气相色谱-串联质谱(gas chromatography-tandem mass spectrometry,GCMS/MS)测定。结果表明:在质量浓度0.005~0.2 mg/kg范围内,21种杀菌剂与对应的峰面积间呈良好的线性关系;在0.01、0.05和0.1 mg/kg 3个添加水平下,21种杀菌剂的平均回收率均在70%~122%之间,RSD<20%。该方法适用于草莓中21种杀菌剂残留的快速、高效和准确分析。In order to determine 21 fungicides（spiroxamine, metalaxyl, tetraconazole, nitrothalisopropyl, cyprodinil, penconazole, fluopyram, triflumizole, mepanipyrim, E-metominostrobin, imazalil,myclobutanil, kresoxim-methyl, cyflufenamid, trifloxystrobin, propiconazol, fenhexamid,epoxiconazole, fenamidone, boscalid, azoxystrobin） in Fragaria ananassa, the sample preparation method and GC-MS/MS analysis parameters were optimized. The sample was first extracted by acetonitrile. During the DSPE procedure, PSA and GCB were used as sorbents. After concentrated, the extract was dissolved by a mixed solvent V（hexane）：V（acetone） = 9：1. The extract was then determined with GC-MS/MS. Linear relationships between peak area and mass concentration of 21 fungicides were obtained in the range of 0.005-0.2 mg/kg. When the spiked levels were 0.01, 0.05 and 0.1mg/kg, the average recoveries all ranged from 70 to 122% with RSD 〈20%（n = 5）. This method is suitable for the rapid and accurate detection of 21 fungicides in F. ananassa.

关 键 词：QUECHERS 气相色谱-串联质谱 草莓 杀菌剂 残留 

分 类 号：S482.2[农业科学—农药学] TQ450.263[农业科学—植物保护]