性激素对宣威XWLC-05肺腺癌细胞PTHrP表达影响的研究  

Study on detecting PTHrP expression and its correlation with sex hormone status in human XWLC-05 lung adenocarcinoma cells

作　　者：龚丽明[1] 黄云超[2] 李鸿钧[3] 王东坤[1] 

机构地区：[1]昆明医科大学第四附属医院心胸外科,云南昆明650021 [2]昆明医科大学第三附属医院,云南昆明650118 [3]中国医学科学院北京协和医学院医学生物研究所,云南昆明650118

出　　处：《云南医药》2017年第1期1-5,共5页Medicine and Pharmacy of Yunnan

基　　金：云南省教育厅科学研究基金项目重点项目(编号:2012Z080);国家自然科学基金资助项目(编号:81000672);云南省科技创新强省计划-国际合作专项基金资助项目(编号:2008CA030)

摘　　要：目的探讨宣威XWLC-05肺腺癌细胞中PTHrP的表达,以及雌雄激素对其表达的影响,为女性肺癌患者PTHrP的表达较男性患者PTHrP的表达高提供细胞学方面的依据,并为下一步研究PTHrP的表达与雌性肺癌裸鼠生存优势间关系而做的动物实验建模做准备。方法采用逆转录多聚酶链反应(RT-PCR)和Western Blot,检测XWLC-05细胞中雌激素受体(ER-α,ER-β),雄激素受体(AR)和甲状旁腺激素相关蛋白(PTHrP)的表达;分别用不同浓度的雌二醇和睾酮处理XWLC-05细胞48h,用实时定量PCR(Real-time quantitative PCR)检测激素干预前后XWLC-05细胞中PTHrPmRNA的表达。结果 XWLC-05细胞中ER-β,AR表达,ER-α不表达,PTHrPmRNA表达;雌二醇对细胞PTHrPm RNA表达无影响,睾酮明显降低了PTHrPmRNA的表达。结论 XWLC-05细胞中ER-β,AR表达,PTHrP表达;性激素可调节PTHrP的分泌,雄激素对PTHrP的表达起负调节作用。Objective This study examined the expression of trrHrP in human XWLC-05 lung adenocarcinoma cells and the impact of estrogen and androgen on the expression of PTHrP. It provided a cytological foundation that the expression of PTHrP in female lung cancer patients was higher than that in male patients. It prepared for further studying the relationship between the expression of PTHrP and the survival advantage of lung cancer in female nude mice. Methods Estrogen receptor （ER- or, ER- 13 ）, androgen receptor （AR） and parathyroid hormone related protein （PTHrP） were detected using RT-PCR and Western Blot in Human XWLC-05 lung adenocareinoma cells. Cells were treated with estradiol and testosterone with from 0.1 to 10 nM for 48 hours prior to detecting the expression of PTHrP using real-time quantitative PCR. Results XWLC-05 cell line expressed estrogen receptor （ER-[3 ） , androgen receptor （AR） and PTHrP based on RT-PCR analyses, but estrogen receptor （ER- ct ） not expressed in the cells. Cells were treated for 48 hours with from 0.1 to 10 nM estradiol,and testosterone. Estradiol had no effect on the expression of PTHrPmRNA. Testosterone significantly reduced the expression of PTHrPmRNA. Conclusion Estrogen receptor （ER-13 ）, androgen receptor （AR） and parathyroid hormone related protein XWLC-05 lung adenocarcinoma cells. Sex negative regulatory role on PTHrP. hormones may regulate the secretion （PTHrP） are expressed in human of PTHrP, in which androgen plays anegative regulatory role on PTHrP.

关 键 词：非小细胞肺癌 XWLC-05肺腺癌细胞 甲状旁腺激素相关蛋白PTHrP 性激素 实时定量PCR 

分 类 号：R734.2[医药卫生—肿瘤]