微生物法生产烷烃研究进展  

Research progress in microbial production of alkanes

作　　者：苏绍洁 郭洪伟[1] 陈宏文[1] 陈国[1] SU Shaojie Guo Hongwei CHEN Hongwen CHEN Guo(School of Chemical Engineering and Key Laboratory of Fujian Province for Biochemical Technology, Huaqiao University Amoy, Fujian 361021, China)

机构地区：[1]华侨大学化工学院,福建省生物化工技术重点实验室,福建厦门361021

出　　处：《工业微生物》2017年第1期56-64,共9页Industrial Microbiology

基　　金：国家自然科学基金项目:磁响应修饰单细胞在MFAB反应器中连续转化甘油制备罗伊氏素的应用基础研究(No.21576108);CBP策略合成木糖醇的米曲霉工程菌构建、途径递进强化和代谢调控机制研究(No.21476093);华侨大学自助科研经费:基于解脂亚洛酵母生理特点的代谢流控制平台构建(16BS103);华侨大学研究生科研创新能力培育计划资助项目;泉州市科技计划重点项目(2014Z109)

摘　　要：烷烃在自然界中广泛存在。它不仅是化石能源的主要组成部分,而且在润滑剂、化妆品、水果保藏、植物防护等方面具有广泛的应用价值。本文概述了天然产烷烃的微生物及其烷烃的天然合成途径及其途径中关键酶催化的作用机理,并对近几年国内外运用代谢工程手段改造微生物使其细胞合成烷烃的研究进展作了介绍。微生物生产烷烃可以通过改造烷烃的天然合成菌株或通过在模式微生物中引入异源烷烃合成途径两种方法来强化。最后文章讨论了微生物法生产烷烃存在的不足和今后研究的方向与展望。Alkanes widely distribute in nature world. It is not only the major components of fossil fuel, but also widely used in flieds of lubricants, cosmetics, fruit preservation, plant protection and so on. In this paper, the wild type alkanesproducing microbial strains including their alkanes synthesis pathways and catalysis mechanism of key enzyme were outlined, and the recent research processes of metabolic engineering with microbial strains for synthesizing alkanes were introduced. Microbial production of alkanes could be achieved either by enhancing alkanes production using wild type alkanes-produeing microbial strains or by heterogeneous introduction of alkanes synthesis pathway into cells of model microbial strains. Finally, the limitation and the future research on biotechnological production of alkanes were discussed.

关 键 词：烷烃 微生物法生产 代谢工程改造 合成途径 

分 类 号：TQ211[化学工程—有机化工]