乙肝前S1抗原的磁性免疫层析检测的影响因素研究  

Influences of magnetic immunochromatographic assay for detection of PreS_1 region of hepatitis B virus surface antigen

作　　者：杜娟[1] 徐晓巍[1] 崔正权 王祎龙[3] 贾鑫明[4] 卢瑛[1] DU Juan XU Xiao-wei CUI Zheng-quan WANG Yi-long JIA Xin-ming LU Ying(Laboratory of Quality ＆ Safety Risk Assessment for Aquatic Products on Storage and Preservation, Ministry of Agriculture, Shanghai Engineering Research Center of Aquatic-Product Processing ＆ Preservation, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306 Nanjing iNanoMax Medtech Limited Company, Nanjing 211100 The Institute for Biomedical Engineering and Nano Science, Tongji University School of Medicine, Shanghai 200092 Department of Immunology, Tongji University School of Medicine, Shanghai 200092, China)

机构地区：[1]上海海洋大学食品学院农业部水产品贮藏保鲜质量安全风险评估实验室上海水产品加工及贮藏工程技术研究中心,上海201306 [2]南京爱纳玛斯医药科技有限公司,南京211100 [3]同济大学医学院生物医学工程与纳米科学研究院,上海200092 [4]同济大学医学院免疫学教研室,上海200092

出　　处：《生物学杂志》2017年第1期11-15,38,共6页Journal of Biology

基　　金：上海市科委"科技创新行动计划"生物医药领域科技支撑项目(No.15441905900);上海市科委工程中心能力提升项目(16DZ2280300)

摘　　要：以免疫磁珠作为检测探针,构建了以血清中前S1抗原为标志物的乙肝检测层析试纸条,并重点分析了磁珠粒径、抗体偶联量、p H值、缓冲液和反应温度对该层析检测的影响作用。研究表明,磁珠的分散稳定性对免疫层析检测有显著的影响,因此,磁珠粒径可以作为一项评判其分散稳定性的质控指标。同时,优化抗体添加量,控制层析样本p H值在6.5~9.0之间,并使用p H值更加稳定的PBS基础缓冲液都可以提高层析试纸条检测乙肝抗原的灵敏度和稳定性。可为高灵敏和高稳定性磁性免疫层析试纸条产品的研发提供理论依据。To establish a rapid assay for HBV,a magnetic immunochromatographic assay（ MICA） for detection of Pre S1 Ag in serum samples of people was developed in our study. The influences of hydrodynamic size of magnetic nanobeads,coupled antibody,p H,basal buffer,reaction temperature on the MICA were analyzed emphatically. Results showed that dispersion stability of magnetic nanobeads monitored by hydrodynamic size had a strong effect on MICA. Moreover,optimal antibody addition,proper p H value of 6. 5 to 9and using PBS with more stable p H as the basal buffer could be beneficial to improve the sensitivity and stability of MICA. The results of this research are expected to provide the theoretical guidance for the future development of MICA.

关 键 词：前S1抗原 血清样本 乙肝检测 免疫层析试纸条 免疫磁珠 

分 类 号：R446.6[医药卫生—诊断学]