DEAE-琼脂糖LB膜的制备与表征  

Preparation and characterization of DEAE-agarose Langmuir-Blodgett films

作　　者：李雯露[1] 孔英俊[2] 康跻耀[2] 高建萍[2] 张贵锋[2] 王明林[1] LI Wen-lu KONG Ying-jun KANG Ji-yao GAO Jian-ping ZHANG Gui-feng WANG Ming-lin(College of Food Science and Engineering, Shandong Agricultural University, Taian 271018 State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, CAS, Beijing 100190, China)

机构地区：[1]山东农业大学食品科学与工程学院,泰安271018 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100190

出　　处：《生物学杂志》2017年第1期89-93,共5页Journal of Biology

基　　金：国家自然科学基金(21306205);广东省自然科学基金(2014A030312013)

摘　　要：由于单分子膜性能独特,受到关注度越来越高,利用LB膜技术制备了二乙氨乙基(DEAE)-琼脂糖单分子层。分析其表面压(π)-面积(A)曲线,崩溃压较高,成膜过程明显。研究浓度对DEAE-琼脂糖成膜情况的影响,确定最佳成膜浓度为0.5 mg/m L。考察配基密度与表面电势的关系,其表面电势随着配基密度的增加而增大。采用原子力显微镜(AFM)和布鲁斯特角显微镜(BAM)对制备的LB膜进行了表征,得出表面膜的表面平整、紧密,厚度约为7.9 nm。DEAE-琼脂糖LB膜制备成功,为以后LB膜技术的广泛应用提供了一种新的材料。Diethylaminoethyl（ DEAE）-Agarose was studied for preparing monolayers based on the Langmuir-Blodgett（ LB） film technology. A high collapse pressure and a standard film forming process were found in the surface pressure（ π）-area（ A） isotherm of DEAE-Agarose. The effect of DEAE-Agarose concentration on the film formation showed that 0. 5 mg / m L was the best. The surface potential increased with rise of the ligand density. DEAE-Agarose LB films were characterized with Brewster angle microscopy（ BAM） and atomic force microscope（ AFM） after being transferred onto silicon surface. The morphology of the film was pretty compact,well structured,and relatively smooth. The thickness was about 7. 9 nm and the roughness was about 2 nm. This result indicated that the DEAEAgarose LB film was successfully prepared and could be an novel material for the fabrication of LB films.

关 键 词：DEAE-琼脂糖 LB膜 原子力显微镜 布鲁斯特角显微镜 表面电势 

分 类 号：S245.9[农业科学—农业电气化与自动化] TB383.2[农业科学—农业工程]