相分离条件下能源草柳枝稷的分离特性研究  

A study on separation characteristics of biorefinery potential switchgrass under phase separation conditions

作　　者：刘汗青[1] 朱璐璐[1] 钱爽[1] 任浩[1] 

机构地区：[1]南京林业大学轻工科学与工程学院,南京210037

出　　处：《中华纸业》2017年第4期38-42,共5页China Pulp & Paper Industry

基　　金：南京林业大学杰出青年基金(NLJQ2015-05);2016江苏省大学生创新课题(201610298058Z)

摘　　要：讨论相分离条件下能源草柳枝稷及COMT转基因后培育的柳枝稷的分离特点及分离产物木质素酚和碳水化合物的特征。通过木材化学的基本分析手段,在掌握了转基因和非转基因柳枝稷基本化学成分的基础上,用红外光谱(FTIR)、凝胶色谱(GPC)、氢谱、二维核磁共振谱(2D-HSQC NMR)等重点比较了相分离后木质素酚产物的结构特征,主要链接方式的含量以及S/G比等;同时,用水相凝胶色谱比较研究了转基因前后柳枝稷的碳水化合物在酸性条件下的水解程度。结果表明,转基因前后的柳枝稷均保留了木质素的基本骨架特征,此外,通过C O MT转基因的方法有效地控制了木质素中S结构单元的数量,转基因柳枝稷的S/G比远低于非转基因柳枝稷,而且C O MT转基因植物的特征核磁信号在通过相分离诱导的木质素产物中有明确表达;碳水化合物在仅20min的相分离处理中得到较充分的水解,碳水化合物中既有大量的分子量为180~380的低分子糖存在,同时还有分子量超过2万的水溶性高分子糖,这些糖溶液将为进一步的生物质精炼提供很好的原料。On the background of the improvement and utilization of energy plants, the fusion of biological genetic engineering and materials engineering becomes very important. It is a key problem to successfully control lignin structure and performance as well as to efficiently convert carbohydrates into ethanol. Because lignin chemical structure is complex, large-scale industrial use has not been able to realize. From wood chemistry point of view, it is difficult to grasp and control lignin structure and component features in the existing plants, This study intended to use trangenic and non-trangenic switchgrass as raw materials to separate lignin and sugars quantitatively by using phase separation technology, then compare the structure differences of lignin products and conversion efficiency of sugars. The results indicated that both lignophenols from switchgrass before or after trans genenic （COMT） pretreatment retained the basic skeleton of lignin characteristics, in addition, through the COMT process, the amount of S units decreased, the S/G ratio is far lower than the control type switchgrass. After only 20 rain treatment through phase separation, carbohydrates got full hydrolysis, the aqueous GPC data indicated there are large number of low molecular weight （Mw=180-380） sugars and few number of water-soluble high molecular weight （Mw〉20000） sugars. After removing acid, the sugars solution would be a good raw material for further biomass refining.

关 键 词：相分离法 柳枝稷 木质素酚 碳水化合物 

分 类 号：TS721.3[轻工技术与工程—制浆造纸工程] TQ325.7[化学工程—合成树脂塑料工业]