白蒺藜对光损伤小鼠光感受器细胞的保护作用  

Protective effects of tribulus terrestris L on photoreceptors from developing bright light-induced degeneration

作　　者：徐静[1] 卞敏娟[2] 崔金刚[2] 杜宵烨 张腾[2] 陈瑜[2] 顾江萍[1] 

机构地区：[1]华东理工大学药学院,上海市200237 [2]上海中医药大学附属岳阳中西医结合医院,上海中医药研究院中西医结合临床研究所,上海市200437

出　　处：《眼科新进展》2017年第2期110-113,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:81473732);上海市高校特聘教授(东方学者)人才计划[编号:沪教委人(2011)88号];上海市085一流学科建设科技创新支持计划(编号:085ZY1221);上海市教委曙光计划(编号:13SG42)~~

摘　　要：目的探讨白蒺藜对小鼠视网膜光损伤的保护作用。方法采用10 000 lux白炽光光照30 min建立视网膜光损伤模型,将BALB/c小鼠分为正常对照组、模型组和治疗组,每组各6只。光照前0.5 h治疗组腹腔注射白蒺藜水煎液100μL(生药100mg/20 g),正常对照组和模型组腹腔注射等量生理盐水。光照后3 h、7 d分别通过OCT检测小鼠视网膜形态学改变;造模后7 d处死小鼠,取眼球固定、包埋、切片进行HE染色,视紫红质和短波敏感视蛋白(short wave-length sensitivity opsin,M-opsin)的免疫荧光化学染色,观察视网膜病理改变情况。结果正常对照组内外核层结构清楚、界限明显,视网膜视杆细胞内外节排列整齐;模型组视网膜结构层次模糊且外核层变薄,与正常对照组差异显著;治疗组小鼠视网膜结构与正常对照组没有显著差异。与正常对照组比较,模型组视紫红质和M-opsin表达降低,治疗组显著提高了视紫红质和M-opsin的表达。模型组较正常对照组视网膜外核层厚度显著变薄(P<0.05);治疗组较模型组能显著性预防小鼠视网膜外核层变薄(P<0.05)。结论小鼠视网膜光损伤模型造模成功,白蒺藜对光损伤视网膜具有显著的保护作用。Objective To investigate protective effect of tribulus terrestris L（TTL） on photoreceptor in the model of light-induced retinal degeneration.Methods BALB/c mice were exposed to bright light at the intensity of 10 000 lux for 30 minutes to establish the retinal light damage models.The BALB/c mice were divided into normal control group,model group and treatment group,6 cases in each group.TTL decoction was intraperitoneatly administered to mice 30 minutes prior to illumination in the treatment group.Saline vehicle was administered in the normal control group and model group.Photoreceptor protection of TTL was assessed by optical coherence tomography（OCT） at 3 hours and 7 days after illumination.Gross histology and immunohistochemistry approaches were also taken to examine the retinal protection conferred by TTL at 7 days after bright light exposure.Results Compared to normal retinal morphology in the normal control group prominent photoreceptor loss and diminished rod and cone photoreceptors evidenced by attenuated retinal expression of rhodopsin and M-opsin were observed in the model group.In contrast,TTL treatment resulted in significant protection against bright light-induced photoreceptor degeneration and remarkable preservation of rod and cone photoreceptor cells.The outer retinal nuclear layer in the model group was thinner than that in the normal control group（P〈0.05）,but the treatment group was thicker than the model group（P〈0.05）.Conclusion Bright light induces obviously degeneration in photo receptors in BALB/c mice.Moreover,TTL is shown for the first to significantly protect the photo receptors from bright light-induced degeneration.

关 键 词：白蒺藜 视网膜光损伤 光感受器细胞退行性改变 

分 类 号：R778.1[医药卫生—眼科]