转基因马铃薯检测用质粒标准分子研制  

Development of a Reference Plasmid for Genetically Modified Potatoes Detection

作　　者：安娜[1] 董美[2] 李凯[2] 宛煜嵩[2] 金芜军[2] 韩阳[1] 李亮[2] AN Na DONG Mei LI Kai WAN Yusong JIN Wujun HAN Yang LI Liang(Academy of Science, Liaoning University, Shenyang 110036 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

机构地区：[1]辽宁大学生命科学院,沈阳110036 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《中国农业科技导报》2017年第2期124-130,共7页Journal of Agricultural Science and Technology

基　　金：国家转基因生物新品种培育重大专项(2014ZX0801201B)资助

摘　　要：依据全球转基因作物商业化的强势发展态势,转基因马铃薯的普遍商业化已呈大势所趋。马铃薯是世界第四大食用作物,仅次于水稻、小麦和玉米。目前,全球获批商业化种植的转基因马铃薯45种,主要涉及抗虫、抗病毒及品质改良等性状。转基因技术在给人们带来巨大经济效益的同时,也引起了人们的担忧,主要包括食品安全及环境安全问题等,由此转基因生物产品成分的检测及监管的重要性凸显。在转基因检测领域,标准质粒以容易获得、纯度高、成本低与稳定性好等优点逐渐被广泛应用。在调研了所有商业化的转基因马铃薯的重要信息的基础上,针对这些转基因马铃薯的主要外源基因类型,构建含有抗马铃薯甲虫cry3A基因、胭脂碱合成酶启动子P-nos基因、抗马铃薯Y病毒pvycp基因及内标准基因尿苷二磷酸葡萄糖焦磷酸化酶PATA基因的多靶标质粒pB JGMM003。该质粒可用于大部分已批准转化体及部分在研转基因马铃薯的鉴定、检测与监管工作,解决了转基因马铃薯的阳性标准品匮乏等问题,保障了转基因马铃薯相关工作的顺利进行。According to the global vigorous development momentum of genetically modified （GM） crops, GM potato commercialization has become a general trend. Potato is the 4th important food in the world next to rice, wheat and corn. At present, 45 species of GM potatoes have been approved for commercial cultivation in the world, mainly involved in insect-resistant, virus-resistant and quality improvement, etc. traits. GM technology has brought huge economic benefits to people, but at the same time raised great concern, including food safety and environment security, etc.. Therefore, the importance of testing and supervising the components of GM product becomes prominent. Within the area of testing GM crops, standard plasmid with the merits of easy obtaining, high purity, low cost, good stability is widely applied. This paper investigated all important information for transgenic potato commercialization, build up multi-target-directed plasmid pBJGMM003 containing anti-potato beetle cry3A gene, nopaline synthase promoter （P-nos） gene, anti-potato virus Y （pvycp） gene and internal control gene patatin （PATA）. This plasmid can be used for identification, testing and inspection of GM potatoes. Its detection ability had covered most of GM potatoes. The problem of lacking potato positive standard was solved, thus providing guarantee for the smooth progress of GM potato works.

关 键 词：转基因马铃薯 标准质粒 检测 安全监管 

分 类 号：Q789[生物学—分子生物学]