FUT1基因在上海地区5个猪群体内的多态性分析  

Polymorphism Analysis of FUT1 Gene in Five Pig Herds in Shanghai

作　　者：吴华莉[1,2,3] 涂尾龙[1] 曹建国[1,3] 都启晶[4] 常华[5] 羊兆洪 谈永松[1,2,3] 

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海201106 [2]上海市调控生物学重点实验室,上海200241 [3]上海种猪工程技术研究中心,上海201302 [4]青岛农业大学食品科学与工程学院,山东青岛266109 [5]云南农业大学动物科学技术学院,云南昆明650201 [6]淮安洪泰良种猪繁育有限公司,江苏淮安223472

出　　处：《畜牧与饲料科学》2017年第1期18-20,共3页Animal Husbandry and Feed Science

基　　金：上海市科委科技支撑项目(16140900200);上海市调控生物学重点实验室开放课题基金资助项目;苏北科技专项(BN2016074)资助项目

摘　　要：仔猪腹泻和水肿病主要是由肠毒素型大肠杆菌的特定抗原F18的不同血清型引起的。α-1岩藻糖转移酶基因(FUT1)可作为F18介导的黏附呈敏感性或抗性遗传性状的一个候选基因。试验采用PCR-RFLP技术分析FUT1基因多态性,并检测其在5个猪群体:杜洛克猪(77头)、长白猪(47头)、大白猪(144头)、申农猪(72头)、梅山猪(69头)的基因型和基因频率。结果表明,FUT1基因在杜洛克猪、大白猪、申农猪群体内检测出AA、AG和GG3种基因型。在杜洛克群体内的基因型频率为AA(0.16)、AG(0.40)、GG(0.44);在大白猪群体内基因型频率为AA(0.03)、AG(0.40)、GG(0.57);在申农猪群体内基因型频率为AA(0.01)、AG(0.25)、GG(0.74);在长白猪群体内只检测出AG(0.21)和GG(0.79),没有AA抗病型;在梅山猪群体中检测的所有样品为GG型。Enterotoxigenic Escherichia coli F18 （ECFI 8 ） with different serotypes is considered to be the causative agent of edema disease and post weaning diarrhea in piglets. The α1 fucosytransferase （FUT1） gene has been identified as a candidate gene for genetic traits showing sensitivity or resistance to F18 mediated adhesion. In the present study, the genetic variation of FUT1 gene was assessed by using PCR-RFLP technique. The genotypes and gene frequency of FUT1 gene in five pig herds in Shanghai, including Duroc （n=77）, Landrace （n=47）, Large White （n=144）, Shennong （n=72） and Meishan （n=69）, were determined. The results showed that 3 genotypes （AA, AG, GG） ofFUT1 gene were observed in hers of Duroc, Large White and Shennong： in Duroc herds, the genotype frequency of AA, AG and GG was 0.16, 0.40 and 0.44, respectively; in Large White herds, the genotype frequency of AA, AG and GG was 0.03, 0.40 and 0.57, respectively; in Shennong herds, the genotype frequency of AA, AG and GG was 0.01, 0.25 and 0.74, respectively. In Landrace herds, the genotypes of AG and GG were found and their genotype frequency was 0.21 and 0.79 respectively, and the genotype of AA （disease-resistant type） was absent. There was only GG genotype observed in Meishan herds.

关 键 词：α-1岩藻糖转移酶基因 限制性片段长度多态性 抗病性 多态性分析 

分 类 号：S828.2[农业科学—畜牧学]