一株耐盐高效苯酚降解菌的分离、鉴定及其应用  

Separation,identification and application of a halotolerant strain with efficient phenol degradation ability

作　　者：龙秀锋 曾庆缘 田杰伟[1] 彭必雨[1] 田永强[1] 

机构地区：[1]四川大学制革清洁技术国家工程实验室,四川成都610065 [2]成都外国语学校,四川成都611731

出　　处：《中国皮革》2017年第2期35-39,43,共6页China Leather

摘　　要：从新疆盐碱土壤中分离筛选得到一株能在高盐条件下降解苯酚的微生物菌株HNA-14,经16S r DNA序列及系统进化分析,鉴定其为芽孢杆菌属的一株菌。通过对HNA-14的生理生化特征及苯酚降解能力的研究,确定该菌的最适生长温度为30℃,p H值为8.0,Na Cl浓度为7%,且在10%的高盐浓度下,HNA-14能在96h内完全降解浓度为500mg/L的苯酚。并将该菌株成功应用于皮化企业的含苯酚废水处理。One halotolerant strain HNA- 14 with phenol degradation ability was separated from saline-alkali soil in Xinjiang province, and it was identified as Bacillus sp by 16S rDNA sequence and phylogenetic analysis. The physio- logical and biochemical characteristics and phenol degradation ability of HNA-14 were studied. Results indicate that its optimal temperature, pH value and concentration of NaCl for growth are 30℃, 8, and 7%, respectively. The phe- nol degradation test shows that HNA- 14 can completely degrade concentration of 500mg/L phenol within 96h at 10% salinity. Furthermore, HNA-14 has been successfully applied to phenol-containing waste water treatment of leather chemicals factory.

关 键 词：盐碱土壤 鉴定 苯酚降解 耐盐 含酚废水 

分 类 号：X172[环境科学与工程—环境科学]