福瑞鲤对氨氮胁迫的生理响应  

Physiological response of FFRC strain common carp to ammonia stress

作　　者：李利红 袁宏利 LI Li-hong YUAN Hong-li(Fishery Sciences Research Institute of Shanxi Province, Taiyuan 030006, Chin)

机构地区：[1]山西省水产科学研究所,太原030006

出　　处：《淡水渔业》2017年第1期97-100,共4页Freshwater Fisheries

基　　金：山西省青年科技研究基金(2013021022-5)

摘　　要：以福瑞鲤(Cyprinus carpio)为材料,研究不同浓度氨氮(0、10、20和30 mg/L)处理对幼鱼鳃组织中抗氧化系统和Na^+/K^+-ATP酶活力的影响。结果显示,氨氮胁迫6 h诱导H_2O_2含量、总抗氧化能力(T-AOC)和超氧化物歧化酶(SOD)活性迅速显著升高,并在胁迫24 h时达到最高水平。胁迫96 h后,各处理组H2O2含量、SOD活性和总抗氧化能力均低于对照组水平,MDA含量显著增加。氨氮胁迫后,Na^+/K^+-ATP酶基因转录水平和蛋白活性呈先降低后升高又降低的变化趋势,参与胁迫过程中渗透压的调控。研究结果表明,低浓度氨氮胁迫后,福瑞鲤鳃组织通过调节细胞抗氧化能力,提高Na^+/K^+-ATP酶活性,有效改善鱼体的抗氨氮胁迫能力,但是高浓度氨氮胁迫对鳃组织造成氧化损伤。The effects of ammonia-N on antioxidant system and Na^＋/ K^＋-ATPase were studied in the gill of FFRC strain common carp（ Cyprinus carpio L.）. The carp were transferred off the freshwater and exposed to different ammonia-N levels（ 0,10,20 and 30 mg/L）,and each ammonia-N level was randomly triplicate and sampled at 0,6,24,48 and 96 h,respectively. The results showed that with the concentration of ammonia-N increased,the H2O2 content,total antioxidant capacity（ T-AOC） and superoxide dismutase（ SOD） activity enhanced significantly at first 6 h and reached the maximun after 24 h exposure. Then,the H2O2 content,SOD activity and T-AOC decreased,and MDA content increased significantly in all ammonia-N treatment group after 96 h exposure. The gene expression level of Na^＋/ K^＋-ATPase α1 and Na^＋/K^＋-ATPase β1 increased at 24 h,and then decreased to be lower than that of control group after 96 h exposure. Meanwhile,Na^＋/ K^＋-ATPase activities decreased at first 6 h,and then increased gradually and reached the maximum at 48 h,showing the induction of Na^＋/ K^＋-ATPase in response to ammonia-N stress. These results indicated that ammonia-N stress induced changes in antioxidant system and Na^＋/ K^＋-ATPase level,which play a key role in preventing ammonia-N damage in gill cells.

关 键 词：福瑞鲤(Cyprinus carpio) 氨氮 鳃组织 Na^+/K^+-ATP酶 抗氧化能力 

分 类 号：S965.89[农业科学—水产养殖]