去甲斑蝥素对膀胱癌T24细胞增殖和侵袭力影响  

Effect of norcantharidin on proliferation and invasion of T24 cells

作　　者：任丽平[1] 徐松涛[2] 

机构地区：[1]漯河医学高等专科学校药理教研室,河南漯河462002 [2]漯河医学高等专科学校医疗系

出　　处：《中国公共卫生》2017年第2期218-220,共3页Chinese Journal of Public Health

基　　金：河南省基础与前沿技术研究计划项目(152300410177);漯河医学高等专科学校博士基金(2013-DF-002)

摘　　要：目的探讨去甲斑蝥素对膀胱尿路上皮癌T24细胞生物学行为影响。方法用噻唑蓝法检测不同浓度去甲斑蝥素对T24细胞增殖的抑制作用;用Hoechst 33342染色法检测T24细胞凋亡率;Transwll实验检测去甲斑蝥素对T24细胞侵袭力抑制作用;用酶联免疫法检测T24细胞基质金属蛋白酶-2(MMP-2)及血管内皮生长因子(VEGF)表达水平。结果各剂量去甲斑蝥素均能明显抑制T24细胞生长,呈剂量依赖性(P<0.05);与空白对照组比较,去甲斑蝥素4、16μmol/L组T24细胞凋亡率[分别为(16.02±0.75)%、(28.28±1.17)%]明显增加,透膜细胞数[分别为(23.33±1.53)、(16.17±2.47)个]明显下降,差异均有统计学意义(P<0.05);与空白对照组比较,去甲斑蝥素4、16μmol/L组T24细胞MMP-2[分别为(5.04±1.02)、(3.02±1.15)]和VEGF[分别为(0.65±0.09)、(0.48±0.11)]表达水平明显降低,差异有统计学意义(P<0.05)。结论去甲斑蝥素可抑制T24细胞增殖,促进细胞凋亡,降低细胞侵袭能力,抑制细胞MMP-2及VEGF表达。Objective To explore the influence of norcantharidin on biological behavior of T24 bladder urothelial carcinoma（T24） cells. Methods The inhibition of norcantharidin of different dosages on the proliferation of T24 cells was detected with 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenytetrazolium bromide （MTT）. The apoptosis of T24 cells was assessed with Hoechst33342 staining. The inhibition of non cantharidin on the invasion of T24 cells was determined with transwell assay. The expressions of matrix metalloproteinases-2 （ MMP-2 ） and vascular endothelial growth factor （VEGF） were measured with enzyme-linked immunosorbent assay （ELISA）. Results Different dosages of norcantharidin could inhibit the growth of T24 cells significantly,with a dose-effect relation （P 〈0. 05）. The apoptosis rate （16. 02 ± 0. 75% and 28.28 ±1.17% ） of T24 cells exposed to 4 and 16μ mol/L norcantharidin increased significantly but the number of invasion of T24 cells （23.33 ± 1.53 and 16. 17 ±2.47） decreased significantly compared to those of the control group （ all P 〈 0. 05 ）. The expression levels of MMP-2 （ 5.04 ± 1.02 and 3.02 ±1.15 ） and VEGF （ 0. 65 ± 0.09 and 0. 48 ± 0. 11 ）were down-regulated in the T24 cells exposed to 4 and 16 μmol/L norcantharidin （all P 〈 0. 05 ）. Conclusion The proliferation and invasion of T24 cells can be inhibited by norcantharidin and norcantharidin could inhibit expressions of MMP-2 and VEGF in T24 cells.

关 键 词：膀胱尿路上皮癌T24细胞 去甲斑蝥素 基质金属蛋白酶-2(MMP-2) 血管内皮生长因子(VEGF) 

分 类 号：R737.14[医药卫生—肿瘤]