miR-221与miR-222在甲状腺乳头状癌组织中的表达及临床意义  

Expression of miR-221 and miR-222 in Papillary Thyroid Carcinoma Tissues and Its Clinical Significance

作　　者：李桂荣 赵清侠[2] 宋斌[3] 刘捷[4] 张瑾[4] 李世东[4] 韩想利[4] 刘晖[4] 张文[4] 

机构地区：[1]陕西省第四人民医院耳鼻咽喉头颈外科,西安710043 [2]陕西省人民医院手术二部,西安710068 [3]陕西省人民医院普外二科,西安710068 [4]陕西省人民医院耳鼻咽喉头颈外科,西安710068

出　　处：《现代检验医学杂志》2017年第1期41-44,共4页Journal of Modern Laboratory Medicine

基　　金：陕西省科学技术研究发展计划项目(2015SF050)

摘　　要：目的探讨miR-221与miR-222在甲状腺乳头状癌(PTC)组织中的表达及其与临床病理因素之间的关系。方法收集陕西省人民医院耳鼻咽喉头颈外科2015年5月~2016年5月就诊的甲状腺乳头状癌(43例)患者、结节性甲状腺肿(21例)及癌旁正常甲状腺组织(14例)共78例,行real time-PCR检测组织中的miR-221及miR--222,并结合临床病理因素进行分析,对结果进行统计学分析。结果甲状腺乳头状癌组中的miR-221及miR-222相对表达水平(11.54±3.37,10.67±2.45)高于结节性甲状腺肿组(3.21±1.12,2.89±1.23)及正常对照组(2.02±0.76,1.98±0.34),差异有统计学意义(t=3.62,3.25;3.27,3.01,均P<0.05),且与患者的性别、年龄、肿瘤大小等因素无关(P>0.05),而与局部淋巴结转移、肿瘤侵犯包膜、远处转移以及临床分期相关(P<0.05)。结节性甲状腺肿组中的miR-221及miR-222与正常对照组比较差异无统计学意义(t=0.91,0.79,P>0.05)。结论 miR-221及miR-222可作为甲状腺乳头状癌相对特异的分子标志物,对甲状腺乳头状癌的临床诊断及治疗有重要作用。Objective To explore the expressions of miR-221 and miR-222 in papillary thyroid carcinoma （PTC） ,and relativi- ties with clinical pathological features. Methods Samples from patients of PCT （43 cases）, nodular goiter（21 cases）, and para-carcinoma thyroid tissues（14 cases）, 78 cases in total （from 06/2015 to 05/2016, Shaanxi Provincial People＇s Hospital） were collected. Real time-PCR tests were carried out, then analyzed in relation to clinical pathology features, and statistical a- nalysis was used to evaluate the results. Results The expressions of miR-221 and miR-222 were significantly higher in PTC （11. 54±3.37,10.67±2.45） than in nodular goiter （3.21± 1.12,2.89±1.23） and normal thyroid tissue （2.02±0.76, 1.98±0.34） （t= 3.62,3.25 ,3.27,3.01 ,all P〈0.05）, which were significantly related with Regional lymph node metasta- sis, tumor invasion coated,distant metastasis and clinical stage （P〈0.05）,while not related with the gender,age,or size of the tumor of the patients （P〉0. 05） ,and no differences were found in nodular golter and in normal thyroid tissue （t=0.91, 0. 79,P〈0.05）. Conclusion miR-221 and miR-222 could be considered as a specific molecular marker of PTC,may play an important role in the diagnosis and treatment on PTC.

关 键 词：甲状腺乳头状癌 微小核糖核酸221 微小核糖核酸222 病理学 

分 类 号：R736.1[医药卫生—肿瘤] R730.43[医药卫生—临床医学]