烟雾病患者外周血来源内皮祖细胞体外培养的能力探讨  

Study the Ability of Endothelial Progenitor Cells From Peripheral Blood in Patients With Smoke Disease

作　　者：张文高[1] 赵志煌[1] 李亚斌[1] 尹港峰[1] 

机构地区：[1]沧州市中心医院神经外三科,河北沧州161001

出　　处：《中国卫生标准管理》2017年第1期144-145,共2页China Health Standard Management

摘　　要：目的探讨烟雾病患者外周血来源内皮祖细胞体外培养的能力。方法收集近几年我院因脑缺血或脑出血入院的烟雾病患者,定义为实验组,对照组为同期我院健康志愿者。结果 (1)实验组和对照组培养第9 d、12 d增殖速率差异存在统计学意义(P<0.05),实验组和对照组培养第3 d、6 d、12 d、15 d增殖速率差异无统计学意义(P>0.05)。(2)实验组和对照组EPCs迁移细胞数(26.67±2.333)、(29.30±2.679),差异无统计学意义(P>0.05)。(3)实验组和对照组EPCs迁移细胞数(23.12±2.36)、(23.61±2.14),差异无统计学意义(P>0.05)。结论本文推测烟雾病患者中,内皮祖细胞与正常血管存在类似形态与增值、粘附、迁移功能。Objective To investigate the ability of peripheral blood derived endothelial progenitor cells from peripheral blood in patients with smoke disease. Methods In recent years, patients with cerebral ischemia or cerebral hemorrhage admitted to hospital weredefined as experimental group. Control group for the same period in our hospital healthy volunteers. Results（1） experimental group and control group were cultured for ninth days and 12 days. The proliferation rate was different（P〈0.05）. The proliferation rate of the experimental group and the control group was 3 days, 6 days, 12 days and 15 days.（2）there was no differernt in the number of migrating cells in the experimental group and the control group was（26.67 ±2.333）,（29.30 ± 2.679）, and the migration ability was similar（P〈0.05）.（3） there was no differernt in the number of migrating cells in the experimental group and the control group was（23.12 ± 2.36）,（23.61 ± 2.14）（P〈0.05）.Conclusion In this paper, we hypothesized that endothelial progenitor cells and normal vascular endothelial progenitor cells have similar morphology and proliferation, adhesion and migration.

关 键 词：烟雾病 内皮祖细胞 体外培养 

分 类 号：R743[医药卫生—神经病学与精神病学]