马铃薯3个StSnRK2基因启动子的克隆与序列分析  

Cloning and bioinformatic analysis of three StSnRK2 gene promoters in potato(Solanum tuberosum)

作　　者：范阿棋[1,2] 毛娟[1,3] 杨宏羽[1,2] 张俊莲[1,3] 王蒂[1,2] 白江平[1,2] 

机构地区：[1]甘肃省作物遗传改良与种质创新重点实验室,甘肃省干旱生境作物学重点实验室,甘肃兰州730070 [2]甘肃农业大学农学院,甘肃兰州730070 [3]甘肃农业大学园艺学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2016年第6期36-43,52,共9页Journal of Gansu Agricultural University

基　　金：国家自然科学基金项目(31460369);甘肃省自然科学基金项目(1308RJIA131);甘肃省干旱生境作物学重点实验室开放基金项目(GSCS-2012-04)

摘　　要：【目的】克隆马铃薯StSnRK2.1、StSnRK2.2和StSnRK2.4基因的启动子片段,为马铃薯StSnRK2基因表达与功能研究奠定基础.【方法】以马铃薯(Solanum tuberosum L.)品种‘陇薯3号’为试材,采用PCR技术从马铃薯基因组DNA中分离得到了3个SnRK2基因启动子.通过PCR扩增,酶切鉴定,测序,利用PlantCARE和PLACE在线分析软件预测分析所得序列.【结果】获得与预期大小基本一致的目的片段,分别为StSnRK2.1基因启动子、StSnRK2.2基因启动子和StSnRK2.4基因启动子.分析软件预测分析表明,启动子调控序列中除含有典型的真核生物核心启动子外,还含有多个CAAT-box、TATA-box等启动子元件,以及相应的ABRE、DRE/CRT、LTRE等与激素应答、逆境胁迫相关的顺式作用元件.【结论】这些结果预示StSnRK2.1、StSnRK2.2和StSnRK2.4基因调控机制相对复杂,可能参与了ABA,干旱,盐等多种逆境胁迫信号的转导过程,在马铃薯逆境胁迫应答过程中具有重要的功能作用.[ObjectivelStSnRK2. 1 ,StSnRK2. 2 and StSnRK2. 4 gene promoter fragments of potato were cloned by molecular cloning, and it laid the foundation of further studies in potato StSnRK2 gene ex- pression and function. [Method] Potato ＇cv. Longshu 3~ （Solanum tuberosum L. ） was the test material, three SnRK2 gene promoters were cloned from potato genomic DNA by PCR, restriction enzyme, sequen- cing. PlantCARE and PLACE were used for analyzing the obtained sequences. [Result]We obtained the target fragments were consistent with expected size using PCR, restriction enzyme digestion and sequen-cing,and named StSnRK2.1 gene promoter,StSnRK2.2 gene promoter and StSnRK2.4 gene promoter, respectively. PlantCARE and PLACE online analysis software prediction analysis showed that the promot- er regulatory region includes typical eukaryotic core promoter region, in addition to containing multiple CAAT-box,TATA-box. promoter elements and other cis-acting elements were involved in the response to phytohormone and osmotic stresses, such as ABRE, DRE/CRT, LTRE. [Conclusion] StSnRK2. 1, StSnRK2.2 and StSnRK2. 4 gene regulation mechanism is relatively complex, may be involved in the transduction of ABA, drought , salinity and other abiotic stress signal, expression may have relation to po- tato resistance.

关 键 词：马铃薯 StSnRK2基因 启动子 克隆 序列分析 

分 类 号：S531[农业科学—作物学]