The influence of heterogeneity on the analysis of sleep-wake architecture in the single-prolonged stress rats  

作　　者：Ying WANG Hong-sheng BIAN Hang ZHAO Ting-li LI 

机构地区：[1]Heilongjiang University of Chinese Medicine, Harbin 150040, China [2]Da Li University, Dali 671000, China

出　　处：《中国应用生理学杂志》2016年第6期504-508,共5页Chinese Journal of Applied Physiology

基　　金：supported by the Key Project of Science Research Foundation of Yunnan Provincial Department of Education (The study on the sleepimproving effect of Rhizoma,2015Z151)

摘　　要：Objective: To observe the influence of heterogeneity on sleep-wake architecture in single-prolonged stress(SPS) animal model. Methods: SPS rats were subdivided into low responders(LR) and high responders(HR) based on their freezing responses to a novel environment. Sleeping time(ST), awakening numbers(AN), brief awakening numbers(b AN) and frequency distribution of sleep bouts were used as observing indicators, single factor variance analysis combined with Dunnett t test were used to compare the differences between control, exposure, LR and HR groups. Results: We found sleeping time was increased only in HR group. Moreover, awakening numbers and brief awakening number increased in exposure group and HR group during the light phase, but not in LR group. The number of sleep bouts for the ranges of 40-80 s increased obviously in HR group, but not in exposure and LR group. In addition, there were significant correlation between sleep-related parameters and freezing in HR group, but not in LR group. Conclusion: Heterogeneity existed in SPS model in view of different sleep-wake architectures of SPS rats. Rats in HR group exactly mimicked the freezing response and sleep disorders of PTSD. So HR rats were more appropriate to be used as PTSD-like models, especially when studying sleep disorder in PTSD.Objective： To observe the influence of heterogeneity on sleep-wake architecture in single-prolonged stress（SPS） animal model. Methods： SPS rats were subdivided into low responders（LR） and high responders（HR） based on their freezing responses to a novel environment. Sleeping time（ST）, awakening numbers（AN）, brief awakening numbers（b AN） and frequency distribution of sleep bouts were used as observing indicators, single factor variance analysis combined with Dunnett t test were used to compare the differences between control, exposure, LR and HR groups. Results： We found sleeping time was increased only in HR group. Moreover, awakening numbers and brief awakening number increased in exposure group and HR group during the light phase, but not in LR group. The number of sleep bouts for the ranges of 40-80 s increased obviously in HR group, but not in exposure and LR group. In addition, there were significant correlation between sleep-related parameters and freezing in HR group, but not in LR group. Conclusion： Heterogeneity existed in SPS model in view of different sleep-wake architectures of SPS rats. Rats in HR group exactly mimicked the freezing response and sleep disorders of PTSD. So HR rats were more appropriate to be used as PTSD-like models, especially when studying sleep disorder in PTSD.

关 键 词：睡眠时间 异质性 大鼠 应激 结构 单因素方差分析 节段 动物模型 

分 类 号：Q428[生物学—神经生物学] P901[生物学—生理学]