降水变化对红松阔叶林土壤微生物功能多样性的影响  

Effects of precipitation change on soil microbial functional diversity in the primitive Korean pine and broadleaved forests

作　　者：王楠楠[1] 韩冬雪[1] 孙雪[1] 国微 马宏宇 冯富娟[1] 

机构地区：[1]东北林业大学,哈尔滨150040 [2]长白山科学研究院,安图133613

出　　处：《生态学报》2017年第3期868-876,共9页Acta Ecologica Sinica

基　　金：国家自然科学基金项目(41105104);中央高校基本科研业务费专项(2572015EA02);长白山科学研究院开放基金项目(201506)

摘　　要：红松阔叶林生态系统是中国东北地带性顶级植被,具有重要的生态学意义;长白山是研究温带森林对大气降水变化正负反馈的理想地带。以长白山红松阔叶林0—5cm、5—10cm表层土和红松根际土为研究对象,基于Biolog生态平板法,分析了不同降水量条件下土壤微生物功能多样性的变化规律及机制。结果表明,平均颜色变化率(Average well color development,AWCD)随培养时间的延长而增加,在培养初期的表现为降水减少30%样地>降水增加30%>对照样地,培养的末期,AWCD值的变化没有表现出明显的规律性。各降水量条件下根际土土壤微生物的AWCD值均高于表层土。Shannon多样性指数、Simpson优势度指数和McIntosh均匀度指数在不同降水量样地间均未表现出显著的差异,即降水量增减30%的情况下,未对红松林表层土和红松根际土的微生物功能多样性产生显著影响。土壤微生物利用率最高的碳源是氨基类,其次是碳水类和多聚类,其中氨基类和碳水类也是不同降水条件下微生物功能多样性表现出异质性的主要碳源。Global climate change and sustainable development are the two most important challenges worldwide. Patterns of precipitation, includes quantity, intensity and distribution, are expected to change. Subsequently, soil moisture content is expected to followed the precipitation pattern changes, which would affect the aboveground biomass and soil microorganisms activity. The latter would consequently affect the structure and functioning of terrestrial ecosystem. Therefore, it is critical to understand how ecosystem structure and function will respond to the expected changes of precipitation pattern. Many large controlled field experiments modify the precipitation pattern change. Most of those studies have been focusing on species composition, productivity and soil respiration. Soil microorganisms are sensitive to micro-environmental changes, which drive the soil nutrient cycling, as well as ecosystem processes such as soil material conversion and energy flow. Therefore, they have been recognized as sensitive indicator of soil ecosystem. As a consequence, soil microorganisms have been a popular research topic in pedology, and have become an extremely important and relatively quick growing research field. Forest ecosystem can contribute greatly to adjust of global climate change. Broad-leaved Korean pine mixed forest is zonal climax vegetation in northeastern China, and it plays a significant ecological role in this region. We selected Changbai Mountain to study the microbial feedbacks to the precipitation changes. Soil microbial functional diversity and its changes driven by Korean pi precipitation change （30% increase and decrease） were measured in different layer soil （0--5cm, 5--10cm and ne rhizosphere soil）, using the Biolog microplate （AWCD） value were highest in the increased precipitation cultivation, however, it did not have an obvious pattern with Our results showed that the average well color development plots, and lowest in the control plot at the beginning of the precipitation regime at the end

关 键 词：碳源利用 BIOLOG 土壤微生物 功能多样性 土壤水分 

分 类 号：S714.3[农业科学—林学]