2-甲氧基雌二醇对脑梗死大鼠神经功能及神经细胞凋亡的影响  

Neuroprotective effect of 2-methoxyestradiol on neurological function and nerve cell apoptosis in rats with acute cerebral infarction

作　　者：彭静华[1] 金飞[2] 张鸿日[3] 张育德[1] 

机构地区：[1]河南科技大学第一附属医院神经内科,洛阳471003 [2]河南科技大学第一附属医院第一药房,洛阳471003 [3]河南科技大学第一附属医院神经外科,洛阳471003

出　　处：《中华神经医学杂志》2017年第2期139-142,共4页Chinese Journal of Neuromedicine

基　　金：洛阳市青年医学科技创新联合专项资金项目（1503008A-15）

摘　　要：目的探讨2-甲氧基雌二醇（2ME2）对急性脑梗死大鼠神经功能及神经细胞凋亡的影响。方法成年雄性SD大鼠72只按随机数字表法分为假手术组、模型组和2ME2治疗组，每组24只。后2组采用Longa法制作大鼠大脑中动脉闭塞模型，造模后2ME2治疗组给予2ME2（24mg／kg）腹腔注射，连续7d；假手术组及模型组给予等剂量生理盐水注射。造模后7d采用Bederson标准评分进行神经功能缺损评估，采用TUNEL法检测脑组织细胞凋亡．应用Western blotting检测大鼠脑组织中Caspase．3的表达情况。结果2ME2治疗组Bederson标准评分较模型组明显下降[（1．235±0．849）分vs（2．015±0．871）分]，差异有统计学意义（P＜0．05）。与假手术组（7．8±6．9、0．286±0．160）比较，模型组和2ME2治疗组大鼠脑组织中凋亡细胞数、Caspase．3表达明显增加，差异均有统计学意义（P＜0．05）；且2ME2治疗组大鼠脑组织中凋亡细胞数（27．8±6．4）、Caspase．3表达（0．615±0．185）较模型组（34．9±5．9、1．109±0．224）明显减少，差异均有统计学意义（P＜0．05）。结论2ME2可以改善急性脑梗死大鼠的神经功能，减少神经细胞凋亡，具有一定的神经保护作用。Objective To investigate the neuroprotective effect of 2-methoxyestradiol （2ME2） on neurological functions and nerve cell apoptosis in rats with acute cerebral infarction. Methods Seventy-two adult male SD rats were randomly divided into sham-operated group, model group and treatment group （n=24）. Middle cerebral artery infarction models in the later two groups were constructed by Longa method. Rats in treatment group were treated with 2ME2 （24 mg/kg） daily for 7 d, while rats in the sham-operated group and model group with physiological saline. Bederson standard scale was adopted to analyze the nerve function defect 7 d after operation. Caspase-3 protein expression in the rat brain tissues was analyzed by Western blotting and apoptosis index was analyzed by TUNEL. Results As compared with those in the model group （2.015±0.871）, Bederson standard scale scores in the treatment group （1.235±0.849） were significantly lower （P〈0.05）; as compared with the sham-operated group （0.286±0.160, 7.8±6.9）, the model group and treatment group had significantly increased Caspase-3 protein level and apoptosis index （P〈0.05）, while Caspase-3 protein level and apoptosis index in treatment group （0.615±0.185, 27.8±6.4） were significantly lower than those in the model group （1.109±0.224, 34.9±5.9, P〈0.05）. Conclusion 2ME2 can improve the neurological function, reduce the level of apoptosis in rat brain nerve cells and has a protective effect on nerve.

关 键 词：2-甲氧基雌二醇 脑梗死 细胞凋亡 神经功能 

分 类 号：R743.33[医药卫生—神经病学与精神病学]