Musashi2对白血病K562细胞体外侵袭能力的影响  

Effect of Musashi2 on invasion potential of leukemia K562 cells in vitro

作　　者：邵会媛[1] 苗宗玉[1] 张正芳[1] 张贵丽[1] 孙成铭[1] 

机构地区：[1]青岛大学附属烟台毓璜顶医院检验中心,山东烟台264000

出　　处：《中国生物制品学杂志》2017年第2期150-153,156,共5页Chinese Journal of Biologicals

基　　金：山东省自然科学基金培养基金项目(ZR2015PH042);烟台市科技发展计划项目(2015WS012)

摘　　要：目的探讨Musashi2(Msi2)对白血病K562细胞体外侵袭能力的影响。方法将靶向Msi2基因的si-RNA1、siRNA2及阴性对照序列转染K562细胞,同时设空白对照组(未转染的K562细胞)。实时荧光定量PCR法及Western blot法分别检测K562细胞中Msi2基因m RNA转录及蛋白的表达水平;经细胞生长曲线观察细胞体外增殖能力;细胞黏附试验检测K562细胞体外黏附能力;Transwell试验检测K562细胞体外迁移及侵袭能力。结果与阴性对照组及空白对照组比较,Msi2-1及Msi2-2组K562细胞中的Msi2基因m RNA转录及蛋白的表达水平显著下降(P<0.05),体外黏附、迁移、侵袭能力明显减弱(P<0.05)。结论抑制Msi2的表达可显著抑制白血病K562细胞的体外侵袭能力,本实验为进一步研究Msi2在白血病发生发展中的调控机制奠定了基础。Objective To investigate the effect of Musashi2（Msi2）on invasion potential of leukemia K562 cells in vitro.Methods K562 cells were transfected with the si-RNA1, si-RNA2, targeting Msi2 gene, and the negative control sequence respectively, using those untransfected as blank control. The m RNA transcription and protein expression levels of Msi2 in K562 cells were determined by real-time fluorescent quantitative PCR and Western blot respectively. The proliferation potential in vitro of K562 cells was observed by using cell growth curve, while the adhesion ability in vitro by cell adhesion assay, and the migration and invasion potential in vitro by Transwell assay. Results Compared with those in negative and blank control groups, scramble and K562 groups, both the m RNA transcription and protein expression levels of Msi2, as well as the adhesion, migration and invasion abilities in vitro of K562 cells in Msi2-1 and Msi2-2 groups decreased significantly（each P〈0. 05）. Conclusion The inhibition of Msi2 expression inhibited the invasion in vitro of K562 cells signifiantly, which laid a foundation of further study on role of Msi2 in regulation of pathogenesis and progress of leukemia.

关 键 词：Musashi2 K562白血病细胞 体外侵袭能力 

分 类 号：R733.72[医药卫生—肿瘤]