葎草叶蛋白提取工艺与含量测定研究  

Extraction and determination of leaf protein from Humulus Scandens

作　　者：石秀梅[1] 李春彦[1] 赵若晗[2] 

机构地区：[1]牡丹江医学院药学院,黑龙江牡丹江157011 [2]牡丹江医学院临床医学院,黑龙江牡丹江157011

出　　处：《中兽医医药杂志》2017年第1期39-41,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：牡丹江医学院科学技术研究项目:葎草中生物活性成分提取工艺的实验研究(项目编号:ZS201315)

摘　　要：目的:研究葎草叶蛋白的提取工艺及含量测定。方法:采用正交试验法,以浸提温度、浸提时间、浸提液质量分数为实验因素,以叶蛋白得率为指标,优化叶蛋白最佳提取条件,紫外-可见分光光度法测定叶蛋白含量。结果:叶蛋白最佳的提取工艺条件为:浸提液质量分数10%、浸提温度50℃、浸提时间20 min。精密度实验RSD为0.21%,稳定性试验RSD为0.72%,加样回收试验RSD为1.46%。结论:葎草叶蛋白的提取方法简便快捷、精密度较高、稳定性较好、方法可行,叶蛋白得率3.65%。Objective：To discusses the extraction and content of leaf protein from Humulus Scandens.Methods：The orthogonal experiment method was used to extract leaf protein with the protein yield as the index,and the extraction temperature,extraction time and mass fraction as the experimental factors.Uv-vis spectrophotometry was used to measure the content of the leaf protein.Results：The best extraction conditions were as follows：the mass fraction 10%,temperature of 50 ℃ and time of 20 minutes for the extraction of leaf protein from Humulus Scandens.RSD was 0.21%,0.72% and 1.46% in the precision test,the stability test and the recovery test.Conclusion：It is simple and fast to extract leaf protein from Humulus Scandens with high precision,stability and recovery.The yield of the leaf protein is 3.65%.

关 键 词：葎草 叶蛋白 提取工艺 含量测定 紫外-可见分光光度法 

分 类 号：S853.7[农业科学—临床兽医学]