微乳过瘤胃胆碱模型探究及体外效果评定  

Emulsion-based delivery system for choline: encapsulation and release properties in vitro

作　　者：靳纯嘏 刘大鹏[1] 覃定奎[1] 颜陶[1] 任豪[1] 姚军虎[1] 蔡传江[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《饲料工业》2017年第3期52-57,共6页Feed Industry

基　　金：国家科技支撑计划[2012BAD12B02]

摘　　要：通过乳化法构建微乳型过瘤胃胆碱模型(W/O-RPC)并进行体外效果评定,包括:W/O-RPC模型的构建及验证,W/O-RPC模型配方的优化,W/O-RPC模型体外效果评定。结果表明,乳化剂比例、油水比对W/O-RPC氯化胆碱保护率影响极显著(P<0.01),当乳化剂比例高于4.00%、油水比≥55时,W/O-RPC氯化胆碱保护率大于90%;利用体外发酵系统并应用响应面分析法对不同配方的W/O-RPC模型进行优化得到各因素对氯化胆碱释放率的影响顺序为油水比>乳化剂比例,所得最佳配方为乳化剂比例11.01%,油水比82;以瑞信RPC为对照,在人工唾液中W/O-RPC的2 h降解率为9.72%,显著高于瑞信RPC(5.46%,P<0.05),W/O-RPC的24 h降解率为13.18%,与瑞信RPC无显著差异(13.67%,P>0.05),体外发酵系统中W/O-RPC的2 h和24 h降解率分别为44.00%和87.55%,均显著高于瑞信RPC(5.09%和26.10%,P<0.05),瘤胃后消化中W/O-RPC的2 h和24 h降解率分别为28.06%和52.11%,均与瑞信RPC差异显著(12.30%和67.84%,P<0.05)。This research was conducted to fabricate a new emulsion-based microencapsulation system for rumen-protected choline （W/O-RPC）, and evaluate its release properties in vitro. In particular, the following series of experiments were performed： preparation of W/O-RPC（Expt 1）, formula optimization of W/O-RPC （Expt 2）, and the evaluation of W/O-RPC protective effect in vitro（Expt 3）. Expt 1 was designed to investigate the effects of emulsifier concentration（wt%） and oil-to-water ratio on the protec- tion rate of encapsulated choline chloride. The results showed that the protection rate was relatively stable（〉90%） when the emulsifier concentration was above 4% and oil-to-water ratio was below 5 ： 5. In Expt 2, the Central Composite Design （CCD） and Response Surface Methodology（RSM） were em- ployed to investigate the influence of emulsifier eoncentration（wt%） and oil-to-water ratio on the re- lease properties of choline chloride in W/O-RPC. Response surface and contour were graphed with the value of release property of choline chloride in W/O-RPC as response value. Results were as follows： The order of 2 factors＇ effects on response value was O ： W ratio 〉 emulsifier concentration （wt%）. The optimal emulsifier concentration and oil-to-water ratio were 11.01 wt% and 8 ： 2, respectively. Under this condition, the theoretical release rate of choline chloride in W/O-RPC was 81.01%, and the actual value was（81.65±1.03）%. In Expt 3, an in vitro model simulating were used to evaluate the rate and extent of lipid phase digestion, as the rumen digestion conditions well as the protective effect of W/O-RPC. The results are showed as follows：①After the treatment of artificial saliva （2 hours）, the release rate of W/O-RPC was significantly higher（9.72%, P〈0.05） than that of a commercial product （Reashure RPC）. However, there was no significant difference（P〉0.05） between W/O and Reashure RPC after a 24 h digestion of artificial saliva, the release rate

关 键 词：胆碱 过瘤胃技术 乳化 响应面法 酶解消化 

分 类 号：S816.32[农业科学—饲料科学]