miRNA-320d和FoxM1在贲门癌组织中表达相关性及临床价值分析  

Expression correlation and analysis of clinical value of miRNA-320d and FoxM1 in the carcinoma of gastric cardia tissues

作　　者：冯帅豪 高社干[1,2] 陈晓杰[1,2] 刘怡文[1,2] 冯笑山[1,2] 

机构地区：[1]河南科技大学临床医学院,洛阳471003 [2]河南科技大学第一附属医院河南科技大学肿瘤研究所河南省肿瘤表观遗传重点实验室,洛阳471003

出　　处：《安徽医科大学学报》2017年第2期194-198,共5页Acta Universitatis Medicinalis Anhui

基　　金：2016年度河南省科技创新人才计划项目

摘　　要：目的探究miRNA-320d和FoxM1在贲门癌组织中的表达相关性及其潜在临床价值。方法收集60例贲门癌与配对癌旁组织,采用荧光实时定量PCR(qRT-PCR)法检测miRNA-320d表达水平及miRNA-320d的潜在下游调控靶基因FoxM1 mRNA表达水平;采用免疫组化(IHC)及Western blot法验证FoxM1的蛋白表达;随访分析miRNA-320d和FoxM1对60例患者生存期的影响。结果 miRNA-320d在贲门癌组织中表达水平显著低于癌旁组织(P<0.01);FoxM1在贲门癌组织中mRNA及蛋白水平均高表达,且miRNA-320d与FoxM1在核酸表达水平上呈负相关性;Kaplan-Meier生存分析显示,高表达miRNA-320d的贲门癌患者总生存率(OS)高于低表达者(P<0.01);低表达FoxM1的贲门癌患者OS高于高表达者(P<0.01)。结论在贲门癌中FoxM1是miRNA-320d的潜在下游调控靶基因,二者有望作为贲门癌患者的预后分子靶标。Objective To investigate the expression of the miRNA-320d and FoxM1 in the carcinoma of gastric cardia tissues, and their association with the clinical prognosis. Methods 60 cases of cardiac carcinoma tissues and matching tissues adjacent to carcinoma were collected, and quantitative real-time PCR was used to examine the ex- pression of miRNA-320d and potential downstream target gene FoxM1 mRNA. The expression of FoxM1 protein was detected by IHC and Western blot. The influence of miRNA-320d and FoxM1 on 60 cases of survival was studied by follow-up analysis. Results The expression of miRNA-320d in carcinoma of gastric cardia tissues was signifi- cantly lower than that in normal tissues adjacent to carcinoma （P 〈 0.01 ）. The expression of FoxM1 on mRNA and protein levels in cardiac carcinoma tissues was both higher than that in matching tissues adjacent to carcinoma. The expressions of miRNA - 320d and FoxM1 were negatively correlated in nucleic acid level. Kaplan-Meier survival a- nalysis showed that the overall survival （OS） of cardiac carcinoma patients who had high expression of miRNA- 320d was higher than that of low expression （P 〈 0. 01 ）. The OS of cardiac carcinoma patients who had low ex- pression of FoxM1 was higher than that of high expression （P 〈0. 01 ）. Conclusion FoxM1 is the potential down- stream target gene of miRNA-320d in carcinoma of gastric cardia. Both of these are expected as molecular targets in the prognosis of cardiac carcinoma patients.

关 键 词：贲门癌 miRNA-320d FOXM1 预后 

分 类 号：R735.1[医药卫生—肿瘤]