SIRT6在结直肠癌组织中的表达及其临床意义  

Expression and clinical significance of SIRT6 in colorectal cancer

作　　者：齐佳[1] 崔春晖[1] 陈日红[1] 翟端洋 王利峰[2] 谢朗[1] 俞金龙[1] 

机构地区：[1]南方医科大学珠江医院普外科,广州510280 [2]南方医科大学珠江医院消化内科,广州510280

出　　处：《安徽医科大学学报》2017年第2期281-285,共5页Acta Universitatis Medicinalis Anhui

基　　金：广东省自然科学基金(编号:2014A030313295)

摘　　要：目的探讨结直肠癌组织中去乙酰化酶6(SIRT6)的表达及其临床意义。方法采用Western blot和实时荧光定量PCR法分别检测29对结直肠癌及癌旁正常组织中SIRT6mRNA和蛋白的表达水平。免疫组化SP法检测SIRT6蛋白在113例(含前29例)结直肠癌及癌旁正常组织切片中的表达,分析SIRT6的表达与各临床参数之间的相关性。结果结直肠癌组织中SIRT6 mRNA和蛋白水平的表达均明显低于正常组织,差异有统计学意义(P<0.05);SIRT6蛋白在不同的分化程度、浸润深度、淋巴结转移及TNM分期结直肠癌组织的表达差异有统计学意义(P<0.05)。结论SIRT6在结直肠癌中的低表达与结直肠肿瘤的发生发展有关,SIRT6可能是结直肠癌的抗癌蛋白之一,可成为结直肠癌诊断和治疗的新靶标。Objective To explore the expression （CRC）. Methods The expression levels of SIRT6 and the clinical significance of SIRT6 in colorectal cancer protein and mRNA in 29 samples of CRC and paired adjacent normal tissues were tested by Western blot and RT-qPCR. 113 paraffin samples （including the former 29 samples） of CRC and adjacent tissues were detected by immunohistochemistry. The associated of expression levels of SIRT6 protein and clinical parameters were analyzed. Results The expression level of SIRT6 was significantly lower in CRC than in adjacent normal tissues（P 〈 0.05 ）. The expression level of SIRT6 was associated with differentiated degree, depth of invasive lymphatic metastasis and TNM stage （ P 〈 0. 05 ）, whereas was not with other clinical pa- rameters. Conclusion The low expression of SIRT6 is involved in the tumorigenesis and progression of CRC. SIRT6 might play a role as tumor suppressor and can be regarded as a promising diagnostic and therapeutic target for CRC.

关 键 词：SIRT6 结直肠癌 WESTERN BLOT RT-QPCR 免疫组化 

分 类 号：R735.3[医药卫生—肿瘤]