膀胱尿路上皮癌中PTEN甲基化与PI3K/Akt信号通路的关系  

Relationship between PTEN methylation and PI3K/Akt signal pathway in bladder urothelial cell carcinoma

作　　者：尹永华[1] 程文杰[2] 延敏博[2] 夏安[1] 尤洪科[1] 侯尚革[1] 

机构地区：[1]广州医科大学附属深圳沙井医院泌尿外科,广东深圳518104 [2]中山大学附属第五医院泌尿外科,广东珠海519000

出　　处：《中国卫生检验杂志》2017年第2期157-159,共3页Chinese Journal of Health Laboratory Technology

基　　金：深圳市科技创新委员会基金资助项目(JCYJ20140414-160300578)

摘　　要：目的探讨膀胱尿路上皮癌中PTEN基因启动子甲基化与PI3K/Akt信号传导通路的关系。方法应用甲基化特异性PCR MSP、Western Blot方法,检测41例膀胱尿路上皮癌和18例正常膀胱组织中PTEN基因启动子甲基化状态,并进一步分析其与PI3K/Akt信号转导通路的相关性。结果膀胱尿路上皮癌中PTEN基因启动子甲基化率为53.66%(22/41),明显高于正常膀胱组织的0%(0/18),2组间差异有统计学意义(P<0.01);在41例膀胱尿路上皮癌组织中,PTEN甲基化阳性组P-Akt的相对表达量为(1.86±0.13),显著高于阴性组(0.92±0.11),二者差异有统计学意义(P<0.05),而2组间Akt的相对表达量则无明显差异。结论膀胱尿路上皮癌中PTEN基因启动子存在高甲基化状态,且与PI3K/Akt信号转导通路蛋白表达呈正相关。Objective To investigate the relationship between the promoter methylation of PTEN and PI3 K / Akt signal pathway in bladder urothelial cell carcinoma（ BUCC）. Methods The promoter methylation of PTEN and the expression of P- Akt were detected by using Methylation Specific PCR（ MSP） and western blot method in 41 cases of BUCC and 18 normal bladder tissues,and the correlation between them were analyzed. Results PTEN gene promoter methylation rate in BUCC was 53. 66%（ 22 /41）,significantly higher than normal bladder tissues[0%（ 0 /18） ],and there was statistical significance on the difference between the two groups（ P〈0. 01）. In 41 cases of BUCC tissues,the relative expression of P- Akt in the methylation positive tissues was1. 86 ± 0. 13,significantly higher than that of the negative group（ 0. 92 ± 0. 11）,and the difference had statistical significance（ P〈0. 05）,while the relative expression of Akt between the two groups had no obvious difference. Conclusion High methylation of PTEN gene was detected in BUCC,and it was positively correlated with PI3 K / Akt signal transduction pathway.

关 键 词：膀胱尿路上皮癌 PTEN甲基化 PI3K/AKT信号传导通路 

分 类 号：R737.14[医药卫生—肿瘤]