大鼠脊髓背角TRPV4在背根神经节持续受压致神经病理性疼痛中的作用  

The role of TRPV4 in spinal cord dorsal horn in the neuropathic pain in Chronic compression of right side dorsal root ganglion model of rat

作　　者：张晓[1] 曲玉娟[1] 贾磊[1] 滕永波[1] 岳寿伟[1] 

机构地区：[1]山东大学齐鲁医院康复科,济南250012

出　　处：《中国康复医学杂志》2017年第2期156-160,共5页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金面上项目(81372123)

摘　　要：目的:探讨大鼠背根神经节(dorsal root ganglion,DRG)持续受压(chronic compression of right side dorsal root ganglion,CCD)后脊髓背角瞬时感受器电位离子通道4(TRPV4)基因及蛋白变化,明确脊髓背角TRPV4在CCD致神经病理性疼痛中的作用。方法:采用健康成年雄性Wistar大鼠,共36只,随机分为3组,分别为空白对照组、CCD手术组、CCD+钌红组。制备大鼠背根神经节持续受压模型,于术前1天、术后第7天、给药前及给药2h后,测量大鼠机械刺激缩爪反应阈值,观察机械痛阈的变化;利用RT-PCR及Western Blot技术检测各组大鼠手术侧脊髓背角TRPV4基因及蛋白表达的变化。结果:与空白对照组相比,术后第7天,CCD组大鼠术侧机械痛阈值明显下降(P<0.001),同侧脊髓背角TRPV4基因及蛋白表达升高(P<0.05);与给药前相比,给予钌红2h后,术侧机械痛阈值明显升高(P<0.001),同侧脊髓背角TRPV4基因及蛋白表达下降(P<0.05)。结论:CCD后大鼠术侧机械痛阈下降,脊髓背角TRPV4基因及蛋白表达升高;钌红可部分逆转CCD后痛觉过敏,部分降低脊髓背角TRPV4基因及蛋白表达。脊髓背角TRPV4参与CCD后大鼠神经病理性疼痛形成。Objective： To investigate the alternation of transient receptor potential vanilloid 4（TRPV4） protein and gene expression in spinal cord dorsal horn in chronic compression of the dorsal root ganglion model of rats, and the role of TRPV4 in neuropathic pain.Method： Chronic compression of right side dorsal root ganglion（CCD） model was established in 36 healthy adult male Wistar rats and randomly divided into three groups： normal, CCD and CCD ＋ RR（ruthenium red）.At 1 day before and 7 days after operation, before and 2 hours after giving ruthenium red, the paw withdrawal mechanical pain thresholds of right side were taken; The alternation of TRPV4 gene and protein expression in right side spinal cord dorsal horn was detected with RT-PCR and Western-Blot technique.Result： Comparing with the normal group, the mechanical pain threshold of operation side in CCD group significantly decreased（P〈0.001） and the TRPV4 gene and protein expression increased（P〈0.05） in the ipsilateral spinal cord dorsal horn on 7 days after CCD operation. Comparing with the ruthenium red giving before,2 hours after giving, the mechanical pain threshold of operation side increased（P〈0.001） and the TRPV4 gene and protein expression decreased（P〈0.05） in the ipsilateral spinal cord dorsal horn significantly.Conclusion： The mechanical pain threshold of operation side decreased after CCD operation and the TRPV4 gene and protein expression increased in the spinal cord dorsal horn. Ruthenium red can partially inverse hyperalgesia after CCD, and lower the TRPV4 gene and protein expression in spinal cord dorsal horn. The TRPV4 of spinal cord dorsal horn takes part in the formation of neuropathic pain after CCD in rat.

关 键 词：背根神经节 脊髓背角 瞬时感受器电位离子通道 神经病理性疼痛 

分 类 号：R741[医药卫生—神经病学与精神病学] R493[医药卫生—临床医学]