EHD2与E-cadher in在肝细胞肝癌中的表达及其相关性  

Expression and correlation of EHD2 and E-cadherin in hepatocellular carcinoma

作　　者：刘金霞[1] 瞿利帅[1] 陆翠华[1] 倪温慨[1] 倪润洲[1] 

机构地区：[1]南通大学附属医院消化内科,江苏省南通市226001

出　　处：《中国肿瘤临床》2017年第3期118-122,共5页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金项目(编号:81401985)资助~~

摘　　要：目的:研究EHD2、E-cadherin蛋白在肝细胞肝癌中的表达及两者相关性。方法:Western blot法和免疫组织化学法检测肝细胞肝癌及对应的癌旁组织中EHD2与E-cadherin表达情况,分析两者的相关性及与患者临床病理学参数及预后的关系。结果:EHD2、E-cadherin蛋白在肝癌组织中的表达明显低于癌旁组织,且两者的表达水平与肝癌的组织分化程度、静脉侵袭及淋巴结转移密切相关(P<0.05),与年龄、性别、肿瘤大小等参数无相关性。EHD2、E-cadherin低表达与肝癌患者的不良预后显著相关(P<0.05)。结论:EHD2、E-cadherin蛋白表达与肝细胞肝癌的发生发展有关,两者的联合检测有助于肝细胞肝癌的诊断、恶性程度及预后的评估。Objective： To investigate the expression and correlation of EHD2 and E-cadherin in hepatocellular carcinoma （HCC）. Meth- ods： Western blot and immunohistochemistry were used to detect the expression of EHD2 and E-cadherin in HCC specimens and adjacent noncancerous tissues. The correlations of EHD2 and E-cadherin with the clinicopathological characteristics and prognosis of patients were further analyzed using Pearson X2 test and Kaplan-Meier method. Results： EHD2 expression, along with E-cadherin, was markedly reduced in HCC tissues than in adjacent noncancerous tissues. Moreover, EHD2 and E-cadherin expression were correlated with histological grade, microvascular invasion, and lymph node metastasis （P〈0.05）. Kaplan-Meier survival analysis showed that HCC patients with decreased EHD2 and E-cadherin expression had shorter overall survival time than those with higher expression. Conclusion： The abnormal expression of EHD2 and E-cadherin possibly promote HCC. Detection of EHD2 and E-cadherin may be valuable for diagnosing HCC and evaluating malignancy extent and prognosis.

关 键 词：肝细胞肝癌 EHD2 E—cadherin WESTERN BLOT 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]