Spink8基因对EC9706细胞增殖、凋亡及迁移能力的影响  

Effects of Spink8 gene on proliferation,apoptosis and migration of EC9706 cells

作　　者：孙艳[1,2] 张华[3] 封青川[1] 范玉佳[1] 李涛[1] 张玉超[1] 贺颖[1] 郑红[1] SUN Yan ZHANG Hua FENG Qingchuan FAN Yujia LI Tao ZHANG Yuchao HE Ying ZHENG Hong(Department of Medical Genetics and Cell Biology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001 Department of Reproductive Medicine,the First Affiliated Hospital,Henan University of Traditional Chinese Medicine,Zhengzhou 450003 Prepotency Division,Women and Infants Hospital of Zhengzhou,Zhengzhou 450012)

机构地区：[1]郑州大学基础医学院医学遗传学与细胞生物学系,郑州450001 [2]河南省中医药大学第一附属医院生殖医学科,郑州450003 [3]郑州市妇幼保健院优生科,郑州450012

出　　处：《郑州大学学报（医学版）》2016年第5期568-571,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省医学科技攻关计划 201303001

摘　　要：目的:探讨Spink8基因对人食管癌EC9706细胞增殖、凋亡及迁移能力的影响。方法:利用DNA重组技术构建Spink8真核表达重组质粒并转染EC9706细胞,构建稳定表达Spink8的EC9706细胞。取未转染、转染空质粒和稳定表达Spink8的EC9706细胞,采用RT-PCR和Western blot法检测Spink8 mRNA和蛋白,MTT法检测细胞增殖,Annexin V-APC/7-AAD法检测细胞凋亡,并进行Transwell细胞迁移实验。结果:与未转染和转染空质粒组细胞比较,稳定表达Spink8的EC9706细胞增殖受到抑制(P<0.05),增殖抑制率为24.5%;细胞凋亡率增加(P<0.05);Transwell小室穿膜细胞数减少(P<0.05)。结论:Spink8可能是一个新的抑癌基因。Aim： To investigate the effects of Spink8 gene on proliferation, apoptosis and migration of human esophageal cancer cell EC9706.Methods： Eukaryotic expression plasmid pEGFP-Spink8 was established by DNA recombination in vitro, and transfected into EC9706 cells. The expressions of Spink8 mRNA and protein were examined by RT-PCR and Western blot. MTT method was used to detect cell proliferation, Annexin V-APC/7-AAD method was used to detect cell apoptosis, and migration ability was detected by Transwell assay.Results： The plasmid of pEGFP-Spink8 was successfully established. Compared with the EC9706 cells without transfection or transfected with null plasmid, the number of surviving cells transfected with pEGFP-Spink8 decreased（P〈0.05）, and proliferation inhibition rate was 24.5%; the apoptosis rate was significantly increased（P〈0.05）; the number of cells which passed Transwell chamber decreased（P〈0.05）.Conclusion： Spink8 may be a new tumor suppressor gene.

关 键 词：Spink8基因 食管癌细胞 增殖 凋亡 细胞迁移 

分 类 号：R735.1[医药卫生—肿瘤]