过氧化氢对人小梁细胞GRP78和CHOP表达的影响  

Effects of H_2O_2 on expressions of GRP78 and CHOP in human trabecular meshwork cells

作　　者：朱艳[1] 朱玉广[1] 孙宝琪[1] 陈晨[1] 李珺[1] 李霞[1] ZHU Yah ZHU Yuguang SUN Baoqi CHEN Chen LI Jun LI Xia(Ophthalmic Center, the Affiliated Hospital, Weifang Medical University, Weifang 261053)

机构地区：[1]潍坊医学院附属医院眼科中心,潍坊261053

出　　处：《郑州大学学报（医学版）》2016年第5期651-653,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：山东省卫生厅科技计划资助项目 2015WS0047

摘　　要：目的:观察H_2O_2对人小梁细胞(HTCs)内质网应激相关蛋白GRP78、CHOP及其mRNA表达的影响。方法:实验分为对照组、H_2O_26 h组、H_2O_212 h组和H_2O_224 h组,分别以600μmol/L H_2O_2作用于HTCs 0、6、12及24 h后,采用Real-time PCR和Western blot检测细胞中CHOP、GRP78 mRNA和蛋白的表达。结果:对照组无GRP78和CHOP的表达;H_2O_2处理后HTCs GRP78、CHOP mRNA及蛋白的表达水平均升高,且随着H_2O_2作用时间的延长,GRP78、CHOP mRNA及蛋白的表达水平升高(P均<0.05)。结论:H_2O_2可诱导HTCs发生内质网应激,从而参与HTCs的氧化损伤。Aim： To observe the effects of H2O2 on expressions of endoplasmic reticulum stress（ERS）markers GRP78 and CHOP in human trabecular meshwork cells（HTCs）,so as to explore the role of ERS in the oxidative damage of HTCs.Methods： The experiment included control group, H2O2 6 h group, H2O2 12 h group and H2O2 24 h group, and HTCs were treated with 600 μmol/L H2O2 for 0,6,12 and 24 h,respectively. The expressions of GRP78 and CHOP mRNA and protein were determined by Real-time PCR and Western blot, respectively.Results： Compared with control group, the expression levels of GRP78 and CHOP mRNA and protein in various H2O2 treatment groups increased; the expressions levels of GRP78 and CHOP mRNA and protein tended to increase along with the prolongation of H2O2 treatment（P〈0.05）.Conclusion： Exogenous H2O2 could induce ERS in HTCs,which is involved in the oxidative damage of HTCs.

关 键 词：过氧化氢 氧化应激 人小梁细胞 内质网应激 

分 类 号：R775.1[医药卫生—眼科]