CCR9对乳腺癌细胞MCF-7增殖和侵袭的影响  

Effects of CCR9 on proliferation and invasion of breast cancer MCF-7 cells

作　　者：刘肖[1] 金晓娟[1] 赵钊[2] LIU Xiao JIN Xiaojuan ZHAO Zhao(Clinical LabOratory, The 117 Hospital of PLA, Hangzhou 310012, China Clinical Laboratory, Zhejiang Provincial People＇s Hospital, Hangzhou 310014, China)

机构地区：[1]解放军第一一七医院检验科,浙江杭州310012 [2]浙江省人民医院检验科,浙江杭州310014

出　　处：《中国现代医生》2016年第35期28-30,35,F0003,共5页China Modern Doctor

基　　金：浙江省自然科学基金资助项目(LQ14H160016)

摘　　要：目的探讨人CCR9基因在乳腺癌细胞中的生物学功能。方法取扁桃体标本并逆转录为c DNA,扩增CCR9基因,连接至pE GFP-N2质粒,脂质体感染人MCF-7乳腺癌细胞。免疫印迹证实CCR9基因在MCF-7乳腺癌细胞表达。CCK-8方法检测MCF-7乳腺癌细胞增殖。结果免疫印迹证实构建pE GFP-N2-CCR9质粒及CCR9基因在MCF-7乳腺癌细胞中高效稳定表达,且CCR9基因能促进MCF-7乳腺癌细胞过度增殖和侵袭(P<0.05或P<0.01)。结论 CCR9基因促进MCF-7乳腺癌细胞增殖和侵袭。Objective To explore the biological function of human CCR9 gene in breast cancer cells. Methods Tonsil samples were taken and reverse-transcripted into cDNA. CCR9 gene was amplified, and was ligated to pEGFP-N2 plasmid. MCF-7 human breast cancer ceils were infected by liposome. Immunoblotting confirmed the expression of CCR9 gene in MCF-7 breast cancer cells. CCK-8 assay was used to detect the proliferation of MCF-7 breast cancer cells. Results Western blot confirmed the construction of pEGFP-N2-CCR9 plasmid and efficient and stable expression of CCR9 gene in MCF-7 breast cancer cells. CCR9 gene could promote the over-proliferatioin and invasion of MCF-7 breast cancer cells（P〈0.05 or P〈0.01）. Conclusion CCR9 gene promotes the proliferation and invasion of MCF-7 breast cancer cells.

关 键 词：CCR9 增殖 侵袭 乳腺癌细胞 

分 类 号：R737.9[医药卫生—肿瘤]