结直肠癌中NDRG2基因的表达及临床意义  

EXPRESSION OF NDRG 2 IN COLORECTAL CARCINOMAS AND ITS CLINICAL SIGNIFICANCE

作　　者：卢佳佳[1] 杨峥[1] 贾淑芹[2] 苏乌云[3] 刘彩霞[3] LU Jia-jia YANG Zheng JIA Shu-qin et al(Inner Mongolia Medical University Hohhot 010059 China)

机构地区：[1]内蒙古医科大学,内蒙古呼和浩特010059 [2]北京大学肿瘤医院暨北京市肿瘤防治研究所 [3]内蒙古医科大学附属医院

出　　处：《内蒙古医科大学学报》2016年第4期283-287,共5页Journal of Inner Mongolia Medical University

基　　金：内蒙古自然科学基金(2013MS1189)

摘　　要：目的:探讨NDRG 2基因在结直肠癌组织中mRNA的表达情况及其与临床病理特征之间的关系。方法:通过实时定量PCR检测68例结直肠癌组织及其配对癌旁组织中NDRG 2 mRNA的表达情况,同时完成5a随访,进而分析病人临床病理特征与术后生存率之间的关系。结果:(1)NDRG 2在68例结直肠癌组织中的mRNA表达量明显低于其配对癌旁组织(P<0.01),差异有统计学意义;(2)TNM分期早(TNM 1)的结肠癌组NDRG 2 mRNA的表达量高于分期晚(TNM 2/3/4)的结肠癌组(T1 VS.T2+3+4,P=0.04);而其余临床病理特征对于结直肠癌组NDRG 2 mRNA表达量的影响无显著差异性(P>0.05);(3)NDRG 2表达水平与结直肠癌病人生存率未见明显相关性(P>0.05)。结论:NDRG 2基因参与结直肠癌的发生发展过程,但具体机制尚未明确。NDRG 2在核酸层面的表达与部位、分化程度、浸润深度、Dukes分期、淋巴结侵犯、术前干预、复发及转移均无明显关系,仅与TNM分期有关,这为进一步探讨结直肠癌的分子诊断及治疗提供理论依据。Objective： To explore the mRNA expression of NDRG 2 which is considered as candidate tumor-suppressor genes in colorectal carcinoma and the relationship with clinical pathological features. Methods： NDRG 2 mRNA levels were examined by real-time quantitative PCR in a cohort of68 human colorectal carcinomas and 68 paired normal tissues. The correlation between NDRG 2 mRNA expression with clinicopathological features and survival followed up for 5 years after surgery was analyzed. Results：（ 1） NDRG 2 mRNA levels were found lower in tumor samples than in the normal tissues（ P〈0. 01）;（ 2） The mRNA expression level of NDRG 2 was higher in patients with TNM 1 compared with TNM 2,3 and 4（ T 2,3 and 4 compared to TNM 1 P = 0. 04）,but there was no significant differences of NDRG 2 mRNA expression between different groups of location differentiation invation Dukes-staging lymph node metastasis preoperation intervention,recurrence and distant metastasis（ P〈0. 05）;（ 3） The expression levels of NDRG 2 were no statistical significance with patients survival.Conclusion： NDRG 2 plays an important part in the progress of colorectal tumor development,invasion or metastasis with unclear mechanism. NDRG 2 transcript levels revealed significant differences between varying TNM staging,which provides the guiding for the clinical diagnosis and prognosis in future.

关 键 词：结直肠癌 NDRG2 实时定量PCR 

分 类 号：R735.3[医药卫生—肿瘤]