理冲生髓饮中多糖及水溶性蛋白质的质量分数测定  

Determination of polysaccharide content and protein content in Lichong Shengsui Drink

作　　者：王艳宏[1] 王超[1] 尚冬雪[1] 李洋[1] 赵永伟[1] 韩凤娟[2] WANG Yan-hong WANG Chao SHANG Dong-xue LI Yang ZHAO Yong-wei HAN Feng-juan(Department of Pharmacology, School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China First Gynecological Department, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, China)

机构地区：[1]黑龙江中医药大学药学院药剂学教研室,哈尔滨150040 [2]黑龙江中医药大学附属第一医院妇科一科,哈尔滨150040

出　　处：《哈尔滨商业大学学报（自然科学版）》2016年第4期399-402,414,共5页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：国家自然科学基金项目(81273788)

摘　　要：建立理冲生髓饮中多糖及水溶性蛋白质质量分数测定的方法.测定理冲生髓饮多糖对葡萄糖的换算因子,利用苯酚-硫酸法测定多糖的质量分数;采用考马斯亮蓝G-250染色法测定理冲生髓饮中可溶性蛋白质的质量分数.多糖标准曲线方程为A=12.839 C-0.008 5（r=0.999 3）,蛋白质标准曲线方程为A=1.271 C-0.033 1（r=0.999 2）,两者分别在0.01～0.1、0.1～1.0 g/L有良好的线性关系.测得理冲生髓饮中多糖质量分数为8.53%,水溶性蛋白质质量分数为0.35%.该两种方法操作简便、灵敏、快速、准确,重复性好,可用于理冲生髓饮中多糖、水溶性蛋白质的质量分数测定.To establish a method for the determination of polysaccharide and water- soluble protein in Lichong Shengsui Drink（ LCSSD）,the conversion factor of LCSSD polysaccharide to glucose was obtained with refined polysaccharide,and the content of LCSSD polysaccharide was determined by using phenol- sulfuric method. The content of protein was determined by using the dying method with Coomassie brilliant blue G- 250. The polysaccharide standard curve equation was A = 12. 839 C- 0. 008 5（ r = 0. 999 3） and protein standard curve equation was A = 1. 271 0 C- 0. 033 1（ r = 0. 999 2）. Respectively the linear range was 0. 01 ~ 0. 1 g / L and 0. 1 ~ 1. 0 g / L. The content of polysaccharide was 8. 53%,and the content of protein was 0. 35%. The method is simple,fast,accurate and reproducible,and it should be helpful for the determination of polysaccharide and water- soluble proteins in LCSSD.

关 键 词：理冲生髓饮 多糖 水溶性蛋白质 质量分数测定 

分 类 号：R286[医药卫生—中药学]